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Diapause
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===Preparation=== The preparation phase usually follows the induction phase, though insects may go directly from induction to initiation without a preparation phase.<ref name="Kostal" /> During this phase, insects accumulate and store molecules such as [[lipids]], [[proteins]], and [[carbohydrates]]. These molecules are used to maintain the insect throughout diapause and to provide fuel for development following diapause termination. Composition of the [[cuticle]] may be altered by changing [[hydrocarbon]] composition and by adding [[lipids]] to reduce water loss, making the organism resistant to desiccation.<ref>{{cite journal |last1= HEGDEKAR |first1= B. M. |year= 1979 |title= Epicuticular Wax Secretion in Diapause and Non-diapause Pupae of the Bertha Army worm |journal= Annals of the Entomological Society of America |volume= 72 |issue= 1 |pages= 13β15|doi= 10.1093/aesa/72.1.13 }}</ref> Diapausing [[pupa]]ria of the [[flesh fly]], ''Sarcophaga crassipalpis'', increase the amount of cuticular [[hydrocarbons]] lining the puparium, effectively reducing the ability of water to cross the cuticle.<ref>{{cite journal |last1= Yoder |first1= J.A. |last2= Denlinger |first2= D.L. |last3= Dennis |first3= M.W. |last4= Kolattukudey |first4= P.E. |year= 1992 |title= Enhancement of diapausing flesh fly puparia with additional hydrocarbons and evidence for alkane biosynthesis by a decarbonylation mechanism |journal= Insect Biochemistry and Molecular Biology |volume= 22 |issue= 3 |pages= 237β243 |doi=10.1016/0965-1748(92)90060-r|bibcode= 1992IBMB...22..237Y }}</ref>
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