Editing Differential centrifugation (section)

== Differences between differential and density gradient centrifugation ==

The difference between differential and density gradient centrifugation techniques is that the latter method uses solutions of different densities (e.g. [[sucrose]], [[Ficoll]], [[Percoll]]) or gels through which the sample passes. This separates the sample into layers by relative density, based on the principle that molecules settle down under a centrifugal force until they reach a medium with the density the same as theirs.<ref>{{Cite web |last=Aryal |first=Sagar |date=2023-05-23 |title=8 Types of Centrifugation with Principles and Uses |url=https://microbenotes.com/centrifugation-principle-types-and-applications/ |url-status=live |archive-url=http://web.archive.org/web/20240530103856/https://microbenotes.com/centrifugation-principle-types-and-applications/ |archive-date=2024-05-30 |access-date=2024-12-17 |website=Microbe Notes |language=en-US}}</ref> The degree of separation or number of layers depends on the solution or gel. Differential centrifugation, on the other hand, does not utilize a density gradient, and the centrifugation is taken in increasing speeds. The different centrifugation speeds often create separation into not more than two fractions, so the supernatant can be separated further in additional centrifugation steps. For that, each step the centrifugation speed has to be increased until the desired particles are separated. In contrast, the density gradient centrifugation is usually performed with just one centrifugation speed.<ref>{{cite journal |last1=Yu |first1=Li-Li |last2=Zhu |first2=Jing |last3=Liu |first3=Jin-Xia |last4=Jiang |first4=Feng |last5=Ni |first5=Wen-Kai |last6=Qu |first6=Li-Shuai |last7=Ni |first7=Run-Zhou |last8=Lu |first8=Cui-Hua |last9=Xiao |first9=Ming-Bing |title=A Comparison of Traditional and Novel Methods for the Separation of Exosomes from Human Samples |journal=BioMed Research International |date=2018 |volume=2018 |pages=1–9 |doi=10.1155/2018/3634563 |pmid=30148165 |language=en |pmc=6083592|doi-access=free }}</ref>