Editing Entner–Doudoroff pathway (section)

=== Conversion of glucose to glucose-6-phosphate   ===
The first step in ED is phosphorylation of glucose by a family of enzymes called [[hexokinase]]s to form [[glucose 6-phosphate]] (G6P). This reaction consumes ATP, but it acts to keep the glucose concentration low, promoting continuous transport of glucose into the cell through the plasma membrane transporters. In addition, it blocks the glucose from leaking out – the cell lacks transporters for G6P, and free diffusion out of the cell is prevented due to the charged nature of G6P. Glucose may alternatively be formed from the [[phosphorolysis]] or [[hydrolysis]] of intracellular starch or glycogen.

In [[animal]]s, an [[isozyme]] of hexokinase called [[glucokinase]] is also used in the liver, which has a much lower affinity for glucose (K<sub>m</sub> in the vicinity of normal glycemia), and differs in regulatory properties. The different substrate affinity and alternate regulation of this enzyme are a reflection of the role of the liver in maintaining blood sugar levels.

''Cofactors:'' Mg<sup>2+</sup>