Editing Intracellular pH (section)

=== Microelectrode ===
The microelectrode method for measuring pH<sub>i</sub> consists of placing a very small electrode into the cell’s cytosol by making a very small hole in the plasma membrane of the cell.<ref name=":1" /> Since the microelectrode has fluid with a high H+ concentration inside, relative to the outside of the electrode, there is a potential created due to the pH discrepancy between the inside and outside of the electrode.<ref name=":3" /><ref name=":1" /> From this voltage difference, and a predetermined pH for the fluid inside the electrode, one can determine the intracellular pH (pH<sub>i</sub>) of the cell of interest.<ref name=":1">{{cite book | vauthors = Loiselle FB, Casey JR | title = Membrane Transporters in Drug Discovery and Development | chapter = Measurement of Intracellular pH | volume = 637 | pages = 311–31 | date = 2010 | pmid = 20419443 | doi = 10.1007/978-1-60761-700-6_17 | isbn = 978-1-60761-699-3 | series = Methods in Molecular Biology }}
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