Editing Please Twins! (section)

===From ''Please Teacher!''===
Some of the ''[[Please Teacher!]]'' characters return in various supporting roles.

;{{Nihongo|Ichigo Morino|森野 苺|Morino Ichigo}}
:{{Voiced by|[[Yukari Tamura]]|[[Julie Ann Taylor]]}}
:Ichigo has changed a lot since the events of ''Please Teacher!''. She has become president of the student council, described in the first novel as the most effective in the school's history, and somewhat perversely enjoys getting dirt on other students. Ichigo usually avoids male companionship though, in fact, she has normal desires in this regard. This is partly due to a unique disease where she was put into a "standstill", a form of metabolic stasis similar to a coma, for six years, which stopped her aging process. The disease can be triggered by psychological stress; hence her deadpan, phlegmatic personality is a counter to prevent its onset, a stronger personality trait in ''Twins'' than in ''[[Please Teacher!]]''. She has also added a quiet "heh heh heh" to the end of many of her statements, as if she were privately enjoying a joke. In effect, she appears to be a grade-schooler when in fact she is much older when the story begins. Most of the details of her life are revealed in ''Please Teacher!'' and are not mentioned in this story. In the ''Twins'' anime series, there was no mention of her growth standstill, while the light novels make only one vague passing allusion. In the novels, Ichigo and Matagu Shido have a history, which Morino dislikes and guards against.

;{{Nihongo|Matagu Shido|四道 跨|Shidō Matagu}}
:{{Voiced by|[[Hiroaki Miura]]|[[Tony Schnur]]}}
:Matagu is a senior to the main characters and tends to come off as an incestuous pervert, though he is otherwise good-natured. His appearances tend to be for comic relief. He also has a sister complex on Haruko, possibly due to him having no luck with women. At the end of this series, he is in the hospital after being haunted by the ghost in the countryside and he has no visitors.

;{{Nihongo|Mizuho Kazami|風見 みずほ|Kazami Mizuho}}
:{{Voiced by|[[Kikuko Inoue]]|[[Bridget Hoffman]]}}
:Mizuho is employed as a teacher at the school where all the characters attend. She is homeroom teacher of Class 1B, with Maiku and Kosei being her students. She is in fact a half-human [[extraterrestrial life in popular culture|alien]]. She can be somewhat ditzy, but also very open-hearted, compassionate and sociable. In a somewhat nostalgic replay of the situation that led to her and Kei being discovered by the former headmaster, Miina and Karen are forced to hide in the very same sports equipment locker. Marie locates them, and Mizuho transports herself to it covertly to counsel the two girls on the true nature of Maiku's seeming gruffness towards them, asserting that it screens genuine kindness and affection for them. Her half-alien nature is never mentioned in the ''Twins'' anime, and this scene is the only allusion to it in the anime. She is married to Kei Kusanagi which is now an [[open secret]] at the school.

;{{Nihongo|Marie|まりえ|}}
:{{Voiced by|[[Tomoko Kaneda]]|[[Sandy Fox]]}}
:Marie is Mizuho's diminutive assistant and companion. A quasi-organic systems link to the operating system controlling Kazami's cloaked and submerged starship and its transporter functions, 'he' has been nicknamed "Fushigi-chan" ("Little Wonder" in English) by Karen for an ability to materialize almost anywhere, often after being summoned without any seeming communications device employed. 'He' makes occasional appearances in the neighborhood, at school and the protagonist's shared residence. As 'his' master Kazami does with "Pochy", 'he' enjoys eating "Prech", a snack food based on the real-life snack [[Pretz]], for which he has an insatiable appetite. The anime establishes that Marie's existence is publicly known and accepted (despite 'his' other-worldly appearance and demonstrated abilities such as levitation) by several of the school's students as being a part of the local UFO folklore surrounding the area, thanks to the events of ''[[Please Teacher!]]''. It is commented by Mizuho that Marie is a frequent visitor and overnight guest at the Kamishiro residence, and is seen 'sleeping' on the same futon with the two girls.

;{{Nihongo|Hatsuho Kazami|風見 はつほ|Kazami Hatsuho}}
:Mizuho's mother. She is a very sensual, self-assured, bold but subtly assertive woman. She appears in one chapter of the light novels, hooking onto Maiku at a festival. She does not appear in the anime or the manga.

;{{Nihongo|Kei Kusanagi|草薙 桂|Kusanagi Kei}}
:{{Voiced by|[[Soichiro Hoshi]]|[[Dave Wittenberg]]}}
:Kei is the senior to the main characters who does not speak in the first 12 episodes of the ''Twins'' series. Kei has the same extraordinary disease as Ichigo which caused him to be in a "standstill" for three years. Kei is now 20 in this series. He was covertly married to Mizuho Kazami in the original series, but their marriage is now common knowledge in this series. This is from [[rumor]]s spread around the school in the [[post-credits scene]] of the ''Please Teacher!'' OVA.

;{{Nihongo|Koishi Herikawa|縁川小石|Herikawa Koishi}}
:{{Voiced by|[[Ayako Kawasumi]]|[[Michelle Ruff]]}}
:Koishi is a third-year high school student in this series. She is slender, with short brown hair cut straight across in back. Her parents own the Herikawa Groceries and she assists there. As such, she becomes Miina and Karen's manager after the two of them obtain part-time jobs at the store, thanks to Ichigo's machinations and her longtime friendship with Koishi. She was most of the time behind the counter studying for university entrance exams. She is seen with Miina in episode 11, and in one brief camera shot in EP12 along with Kaede Misumi. In the OVA, she has a conversation with Miina in which she encourages Miina to pursue a romantic relationship with Maiku, then spends the rest of the episode with Ichigo.

;{{Nihongo|Hyosuke Magumo|間雲 漂介|Magumo Hyōsuke}}
:{{Voiced by|[[Mitsuo Iwata]]|[[Kirk Thornton]]}}
:A brash student with dyed blonde hair, Hyosuke aspires to attend college at [[Tokyo University]] and become a professional statesman, much like his brother. Currently going out with Kaede. He only appears in episode 13.

;{{Nihongo|Kaede Misumi|水澄 楓|Misumi Kaede}}
:{{Voiced by|[[Sayaka Ohara]]|[[Rebecca Forstadt]]}}
:Kaede tends to be a rather shy girl most of the time. Her physical characteristics include that she is one quarter Irish, tall (for a Japanese) and with red hair and freckles. Currently going out with Hyosuke. She appears only in episode 13 and at the end of episode 12 in which she and Koishi have a look at the photos that Ichigo took.

;{{Nihongo|Masaomi Yamada|山田 正臣|Yamada Masaomi}}
:{{Voiced by|[[Tomokazu Sugita]]|[[Tony Oliver]]}}
:A quiet male teacher whose hobby is creating human-powered planes, he is in a secret relationship with Koishi. He is the homeroom teacher of Class 1A. Miina, Karen, Haruko, Akina Sagawa and Futaba Mashita are all in his class. In this series, he has shaved his beard and has become popular with the girls as Koishi said he would.

;Nat
:{{Voiced by|[[Chinami Nishimura]]|[[Wendee Lee]]}}
:Nat (Nat-chan in Japanese) is known as the little girl during the summer (both in this anime, and in ''[[Please Teacher!]]''). In this series she is seated on a lifeguard's tower with her dog seated beside her, scanning with binoculars for a boy to fall in love with. She spots Maiku and falls for him. A moment later, however, she sees Karen and Miina with Maiku and she tumbles backward off the tower, dragging her dog by the leash after her, heartbroken.