Editing Tandem repeat (section)

==Biotechnology==
Kang. et al. successfully ''in vitro'' amplified up to 5kb of a sequence containing 36 identical 99bp tandem repeats and a 561bp sequence with 91% AT content using SHARP, which utilizes engineered superhelicases with enhanced processivity and speed.<ref name="reliableamplification">{{cite journal |last1=Kang |first1=Jimin |last2=Rashid |first2=Fahad |last3=Murray |first3=Peter J. |last4=Merino-Urteaga |first4=Raquel |last5=Gavrilov |first5=Momcilo |last6=Shang |first6=Tiantian |last7=Jo |first7=Wonyoung |last8=Ahmed |first8=Arman |last9=Aksel |first9=Tural |last10=Barrick |first10=Doug |last11=Berger |first11=James M. |last12=Ha |first12=Taekjip |author12-link=Taekjip Ha |title=Reliable amplification of highly repetitive or low complexity sequence DNA enabled by superhelicase-mediated isothermal amplification |journal=bioRxiv |date=November 27, 2024 |doi=10.1101/2024.11.27.625726|pmc=11623625 }}</ref> SHARP combines single-stranded DNA binding protein (SSB) and superhelicases with standard PCR reagents to achieve isothermal amplification that mimics biological DNA replication. The method operates at a constant temperature, eliminating the need for thermal cycling, and has shown particular utility in cases where traditional PCR either fails to amplify target sequences or produces unwanted side products.