Editing DNA sequencing (section)

=== Sequencing by hybridization ===
''[[Sequencing by hybridization]]'' is a non-enzymatic method that uses a [[DNA microarray]]. A single pool of DNA whose sequence is to be determined is fluorescently labeled and hybridized to an array containing known sequences. Strong hybridization signals from a given spot on the array identifies its sequence in the DNA being sequenced.<ref>{{cite journal |author3-link=Daniel Kuritzkes | vauthors = Hanna GJ, Johnson VA, Kuritzkes DR, Richman DD, Martinez-Picado J, Sutton L, Hazelwood JD, D'Aquila RT | title = Comparison of Sequencing by Hybridization and Cycle Sequencing for Genotyping of Human Immunodeficiency Virus Type 1 Reverse Transcriptase | journal = J. Clin. Microbiol. | volume = 38 | issue = 7 | pages = 2715–21 | date = 1 July 2000 | pmid = 10878069 | pmc = 87006 | doi = 10.1128/JCM.38.7.2715-2721.2000 }}</ref>

This method of sequencing utilizes binding characteristics of a library of short single stranded DNA molecules (oligonucleotides), also called DNA probes, to reconstruct a target DNA sequence. Non-specific hybrids are removed by washing and the target DNA is eluted.<ref name="Morey">{{cite journal | vauthors = Morey M, Fernández-Marmiesse A, Castiñeiras D, Fraga JM, Couce ML, Cocho JA | title = A glimpse into past, present, and future DNA sequencing | journal = Molecular Genetics and Metabolism | volume = 110 | issue = 1–2 | pages = 3–24 | year = 2013 | pmid = 23742747 | doi = 10.1016/j.ymgme.2013.04.024 | hdl = 20.500.11940/2036 }}</ref> Hybrids are re-arranged such that the DNA sequence can be reconstructed. The benefit of this sequencing type is its ability to capture a large number of targets with a homogenous coverage.<ref name="Qin">{{cite journal | vauthors = Qin Y, Schneider TM, Brenner MP | title = Sequencing by Hybridization of Long Targets | journal = PLOS ONE | volume = 7 | issue = 5 | pages = e35819 | year = 2012 | pmid = 22574124 | pmc = 3344849 | doi = 10.1371/journal.pone.0035819 | editor1-last = Gibas | bibcode = 2012PLoSO...735819Q | editor1-first = Cynthia | doi-access = free }}</ref> A large number of chemicals and starting DNA is usually required. However, with the advent of solution-based hybridization, much less equipment and chemicals are necessary.<ref name="Morey"/>