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Protein engineering
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====Truncated metagenomic gene-specific PCR==== This method generates chimeric genes directly from metagenomic samples. It begins with isolation of the desired gene by functional screening from metagenomic DNA sample. Next, specific primers are designed and used to amplify the homologous genes from different environmental samples. Finally, chimeric libraries are generated to retrieve the desired functional clones by shuffling these amplified homologous genes.<ref name=PoluriBook/>{{page needed|date=May 2017}}
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