Editing Protein engineering (section)

==== ''In vivo'' Homologous Recombination ====
Cloning performed in yeast involves PCR dependent reassembly of fragmented expression vectors. These reassembled vectors are then introduced to, and cloned in yeast. Using yeast to clone the vector avoids toxicity and counter-selection that would be introduced by ligation and propagation in E. coli.<ref name=PoluriBook/>{{page needed|date=May 2017}}