Editing DNA sequencing (section)

=== ''In vitro'' virus high-throughput sequencing ===
A method has been developed to analyze full sets of [[Interactome|protein interactions]] using a combination of 454 pyrosequencing and an ''in vitro'' virus [[mRNA display]] method. Specifically, this method covalently links proteins of interest to the mRNAs encoding them, then detects the mRNA pieces using reverse transcription [[Polymerase chain reaction|PCR]]s. The mRNA may then be amplified and sequenced. The combined method was titled IVV-HiTSeq and can be performed under cell-free conditions, though its results may not be representative of ''in vivo'' conditions.<ref>{{cite journal | vauthors = Fujimori S, Hirai N, Ohashi H, Masuoka K, Nishikimi A, Fukui Y, Washio T, Oshikubo T, Yamashita T, Miyamoto-Sato E | title = Next-generation sequencing coupled with a cell-free display technology for high-throughput production of reliable interactome data | journal = Scientific Reports | volume = 2 | page = 691 | year = 2012 | pmid = 23056904 | pmc = 3466446 | doi = 10.1038/srep00691 | bibcode = 2012NatSR...2..691F }}</ref>