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Coliform bacteria
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==== PCR ==== Amplification of the [[beta-galactosidase]] gene is used to detect coliforms in general, because all coliform organisms produce this compound.<ref name=":1">{{cite journal | vauthors = RomprΓ© A, Servais P, Baudart J, de-Roubin MR, Laurent P | title = Detection and enumeration of coliforms in drinking water: current methods and emerging approaches | journal = Journal of Microbiological Methods | volume = 49 | issue = 1 | pages = 31β54 | date = March 2002 | pmid = 11777581 | doi = 10.1016/S0167-7012(01)00351-7 }}</ref> The [[Amplification of DNA|amplification]] of the beta-D glucuronidase is used to detect ''E. coli,'' or the amplification of their [[verotoxin]] gene(s) to detect verotoxin-producing ''E. coli.''<ref name=":1" /><ref>{{cite journal | vauthors = Holland JL, Louie L, Simor AE, Louie M | title = PCR detection of Escherichia coli O157:H7 directly from stools: evaluation of commercial extraction methods for purifying fecal DNA | journal = Journal of Clinical Microbiology | volume = 38 | issue = 11 | pages = 4108β4113 | date = November 2000 | pmid = 11060076 | pmc = 87549 | doi = 10.1128/JCM.38.11.4108-4113.2000 }}</ref>
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