Editing Endonuclease (section)

==== DNA crosslink repair ====
[[DNA repair|Repair of DNA]] in which the two complementary strands are joined by an [[crosslinking of DNA|interstrand covalent crosslink]] requires multiple incisions in order to disengage the strands and remove the damage.  Incisions are required on both sides of the crosslink and on both strands of the duplex DNA.  In mouse embryonic stem cells, an intermediate stage of crosslink repair involves production of double-strand breaks.<ref name="Hanada2006">{{cite journal |last1=Hanada |first1=K. |last2=Budzowska |first2=M. |last3=Modesti |first3=M. |last4=Maas |first4=A. |last5=Wyman |first5=C. |last6=Essers |first6=J. |last7=Kanaar |first7=R. |date=2006 |title=The structure-specific endonuclease Mus81-Eme1 promotes conversion of interstrand DNA crosslinks into double-strands breaks |journal=The EMBO Journal |volume=25 |issue=20 |pages=4921–4932 |doi=10.1038/sj.emboj.7601344 |pmc=1618088 |pmid=17036055}}</ref>   [[MUS81]]/[[EME1]] is a structure specific endonuclease involved in converting interstrand crosslinks to double-strand breaks in a DNA replication-dependent manner.<ref name="Hanada2006" />  After introduction of a double-strand break, further steps are required to complete the repair process.  If a crosslink is not properly repaired it can block [[DNA replication]].{{citation needed|date=January 2023}}