Editing Forward genetics (section)

=== Post mutagenesis ===
Once mutagenized and [[Genetic screening|screened]], typically a [[complementation test]] is done to ensure that mutant [[phenotypes]] arise from the same genes if the mutations are recessive.<ref name="parsch" /><ref name="fgt">{{cite web |last1=Hunter |first1=Shaun |name-list-style=vanc |title=Forward Genetics Topics |url=http://classes.biology.ucsd.edu/old.web.classes/bicd100.FA05/forward_genetics.doc |url-status=dead |archive-url=https://web.archive.org/web/20141215034249/http://classes.biology.ucsd.edu/old.web.classes/bicd100.FA05/forward_genetics.doc |archive-date=15 December 2014 |access-date=7 November 2014 |publisher=UCSanDiego}}</ref> If the progeny after a cross between two recessive mutants have a wild-type phenotype, then it can be inferred that the phenotype is determined by more than one gene. Typically, the allele exhibiting the strongest phenotype is further analyzed. A genetic map can then be created using [[Genetic linkage|linkage]] and genetic markers, and then the gene of interest can be cloned and sequenced. If many alleles of the same genes are found, the screen is said to be saturated and it is likely that all of the genes involved producing the phenotype were found.<ref name="fgt" />
[[File:Forward genetics steps.png|center|thumb|659x659px|Flowchart of basic steps involved in forward genetics approach.]]