Editing Intracellular pH (section)

=== Fluorescence spectroscopy ===
Another way to measure Intracellular pH (pH<sub>i</sub>) is with dyes that are sensitive to pH, and fluoresce differently at various pH values.<ref name=":4" /><ref>{{cite journal | vauthors = Specht EA, Braselmann E, Palmer AE | title = A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging | journal = Annual Review of Physiology | volume = 79 | pages = 93–117 | date = February 2017 | pmid = 27860833 | doi = 10.1146/annurev-physiol-022516-034055 | pmc = 12034319 }}</ref> This technique, which makes use of fluorescence spectroscopy, consists of adding this special dye to the cytosol of a cell.<ref name=":3" /><ref name=":1" /> By exciting the dye in the cell with energy from light, and measuring the wavelength of light released by the photon as it returns to its native energy state, one can determine the type of dye present, and relate that to the intracellular pH of the given cell.<ref name=":3" /><ref name=":1" />