Editing Protein sequencing (section)

== C-terminal amino acid analysis ==
The number of methods available for [[C-terminal]] amino acid analysis is much smaller than the number of available methods of N-terminal analysis. The most common method is to add [[carboxypeptidase]]s to a solution of the protein, take samples at regular intervals, and determine the terminal amino acid by analysing a plot of amino acid concentrations against time. This method will be very useful in the case of polypeptides and protein-blocked N termini. C-terminal sequencing would greatly help in verifying the primary structures of proteins predicted from DNA sequences and to detect any posttranslational processing of gene products from known codon sequences.