Editing Southern blot (section)

== Limitations ==

# Compared to other tests, southern blot is a complex technique that has multiple steps and these steps require equipment and reagents that are expensive.<ref name=":1" />
# High quality and large amounts of DNA are needed.<ref name=":1" />
# Southern blotting is a time consuming method and can only estimate the size of the DNA since it is a semi-quantitative method.<ref name=":1" />
# It cannot be used to detect mutations at base-pair level.<ref name=":1" />