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Spring peeper
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=== Thermoregulation === Climate plays a major role in the timing of spring peeper breeding: studies have shown a correlation between temperature and the date of first call (when spring peepers start to breed).<ref>{{cite journal |last1=Blaustein |first1=Andrew R. |last2=Belden |first2=Lisa K. |last3=Olson |first3=Deanna H. |last4=Green |first4=David M. |last5=Root |first5=Terry L. |last6=Kiesecker |first6=Joseph M. |date=14 December 2001 |title=Amphibian Breeding and Climate Change |journal=Conservation Biology |volume=15 |issue=6 |pages=1804β1809 |doi=10.1046/j.1523-1739.2001.00307.x |doi-access=free |bibcode=2001ConBi..15.1804B |s2cid=26685121|hdl=2027.42/75127 |hdl-access=free }}</ref><ref name="Gibbs-2001">{{Cite journal |last1=Gibbs |first1=James P. |last2=Breisch |first2=Alvin R. |date=2001 |title=Climate Warming and Calling Phenology of Frogs near Ithaca, New York, 1900β1999 |journal=Conservation Biology |volume=15 |issue=4 |pages=1175β1178 |doi=10.1046/j.1523-1739.2001.0150041175.x |jstor=3061337 |bibcode=2001ConBi..15.1175G |s2cid=86182135 }}</ref> Though the precise factors affecting breeding timing are complex, there has been a trend towards earlier breeding as average temperatures have increased since the early 20th century.<ref name="Gibbs-2001" /><ref name="Lovett2013" /> Another impact of temperature is the duration of mating calls. There is a negative relationship between the length of mating calls and throat temperature. However, male spring peepers with superior calling frequencies are positively related to throat temperature. The temperature of the surrounding environment of spring peepers also plays a role in the rate of calls, which is positively associated with the success of males during the mating and breeding period, showing that increasing site and throat temperatures result in increasing dominant frequency.<ref name="ReferenceA">{{Cite journal |last1=Sullivan |first1=Brian K. |last2=Hinshaw |first2=Steven H. |date=1990-12-31 |title=Variation in Advertisement Calls and Male Calling Behavior in the Spring Peeper (''Pseudacris crucifer'') |journal=Copeia |volume=1990 |issue=4 |page=1146 |doi=10.2307/1446500 |jstor=1446500 }}</ref> Spring peepers are known to tolerate freezing temperatures by producing a glucose-based cryoprotectant to limit cell shrinkage and prevent cell freezing.<ref name=":1">{{Cite journal |last1=Ethier |first1=Jeffrey P. |last2=Fayard |first2=Aurore |last3=Soroye |first3=Peter |last4=Choi |first4=Daeun |last5=Mazerolle |first5=Marc J. |last6=Trudeau |first6=Vance L. |date=2021-08-27 |title=Life history traits and reproductive ecology of North American chorus frogs of the genus Pseudacris (Hylidae) |journal=Frontiers in Zoology |volume=18 |issue=1 |page=40 |doi=10.1186/s12983-021-00425-w |issn=1742-9994 |pmc=8394169 |pmid=34452622 |doi-access=free }}</ref>
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