Editing DNA synthesis (section)

===RT-PCR===
RT-PCR differs from conventional PCR as it synthesizes cDNA from mRNA, rather than template DNA. The technique couples a reverse transcription reaction with PCR-based amplification, as an RNA sequence acts as a template for the enzyme, reverse transcriptase. RT-PCR is often used to test gene expression in particular tissue or cell types at various developmental stages or to test for genetic disorders.<ref>{{cite book |last1=Bachman |first1=Julia |title=Chapter Two - Reverse-Transcription PCR (RT-PCR) |series=Methods in Enzymology |date=2013 |volume=530 |pages=67–74 |doi=10.1016/B978-0-12-420037-1.00002-6 |pmid=24034314 |url=https://www.sciencedirect.com/science/article/pii/B9780124200371000026}}</ref>