Editing Plant pathology (section)

===Detection===

Ancient methods of leaf examination and breaking open plant material by hand are now augmented by newer technologies. These include [[molecular pathology]] assays such as [[polymerase chain reaction]] (PCR), [[RT-PCR]] and [[loop-mediated isothermal amplification]] (LAMP).<ref name="Mumford-et-al-2006">{{cite journal |last1=Mumford |first1=Rick |last2=Boonham |first2=Neil |last3=Tomlinson |first3=Jenny |last4=Barker |first4=Ian |title=Advances in molecular phytodiagnostics - new solutions for old problems |journal=European Journal of Plant Pathology |date=September 2006 |volume=116 |issue=1 |pages=1–19 |doi=10.1007/s10658-006-9037-0 |pmid=32214677 |pmc=7087944 |bibcode=2006EJPP..116....1M }}</ref> Although PCR can detect multiple molecular targets in a single solution there are limits.<ref name="Mumford-et-al-2006" /> Bertolini et al. 2001, Ito et al. 2002, and Ragozzino et al. 2004 developed PCR methods for multiplexing six or seven plant pathogen molecular products and Persson et al. 2005 for multiplexing four with RT-PCR.<ref name="Mumford-et-al-2006" /> More extensive [[molecular diagnosis]] requires [[PCR array]]s.<ref name="Mumford-et-al-2006" /> The primary detection method used worldwide is [[enzyme linked immunosorbent assay]].<ref>{{cite journal |last1=Venbrux |first1=Marc |last2=Crauwels |first2=Sam |last3=Rediers |first3=Hans |date=8 May 2023 |title=Current and emerging trends in techniques for plant pathogen detection |journal=Frontiers in Plant Science |volume=14 |doi=10.3389/fpls.2023.1120968 |doi-access=free|pmid=37223788 |pmc=10200959 }}</ref>