Editing Cushing reflex (section)

===Experimental setup and results===
Cushing began experimenting once he obtained approval from Kocher. His experimental setup was a modified version of  [[Leonard Erskine Hill|Leonard Hill's]] model to similarly test the effects of brain pressure on sinus pressure, cerebrospinal fluid pressure, [[arterial blood pressure|arterial]] and venous blood pressure.<ref name=Fodstad /><ref name=HillL>{{cite book |author=Leonard Hill |title= Physiology and Pathology of the Cerebral Circulation. |url=https://archive.org/details/cu31924024799110 |publisher= J & A Churchill |location=London |year=1896 }}</ref>  Like Hill, Cushing used dogs for his experiments. To begin, Cushing monitored the caliber and color of cortical vessels by fitting a glass window into the skull of the dog. Intracranial pressure was raised by filling an intracranial, soft, rubber bag with mercury. Cushing recorded the intracranial pressure along with [[blood pressure]], [[pulse rate]], and [[respiratory rate]] simultaneously. This three part effect is commonly referred to as [[Cushing's triad]]. In later experiments performed by [[Angelo Mosso|Mosso]], intracranial pressure was induced by injecting physiological saline into the [[subarachnoid space]] rather than increasing mercury content of an intracranial bag.<ref name=Fodstad />

This research clearly displayed the cause and effect relationship between intracranial pressure and cerebral compression.<ref name=FinkM>{{cite book |author1=Mitchell Fink |author2=Michelle Hayes |author3=Neil Soni |title= Classic Papers in Critical Care.  |publisher= Springer |location=London, England |year=2008 |pages= 89–90 }}</ref>  Cushing noted this relationship in his subsequent publications. He also noted that there must exist a specific regulatory mechanism that increased blood pressure to a high enough point such that it did not create anemic conditions.<ref name="Cushing"/> Cushing's publications contain his observations and no statistical analysis. The sample size of the experiment is also not known.<ref name="FinkM"/>