Editing DNA extraction (section)

== High-molecular-weight DNA extraction method ==
In this method, plant [[Cell nucleus|nuclei]] are isolated by physically grinding tissues and reconstituting the intact nuclei in a unique Nuclear Isolation Buffer (NIB). The plastid DNAs are released from organelles and eliminated with an osmotic buffer by washing and centrifugation. The purified nuclei are then lysed and further cleaned by organic extraction, and the genomic DNA is precipitated with a high concentration of CTAB. The highly pure, high molecular weight [[Genomic DNA|gDNA]] is extracted from the nuclei, dissolved in a high pH buffer, allowing for stable long-term storage.<ref>{{cite journal |last1=Li |first1=Zhigang |last2=Parris |first2=Stephen |last3=Saski |first3=Christopher A. |title=A simple plant high-molecular-weight DNA extraction method suitable for single-molecule technologies |journal=Plant Methods |date=2020 |volume=16 |pages=38 |doi=10.1186/s13007-020-00579-4 |pmid=32190102 |pmc=7071634 |issn=1746-4811|doi-access=free }} [[File:CC-BY icon.svg|50px]]  Text was copied from this source, which is available under a [https://creativecommons.org/licenses/by/4.0/  Creative Commons Attribution 4.0 International License].</ref>