Editing Expression vector (section)

===Yeast===
A yeast commonly used for protein production is ''[[Pichia pastoris]]''.<ref>{{cite journal |title= Recombinant protein expression in Pichia pastoris |vauthors=Cregg JM, Cereghino JL, Shi J, Higgins DR |journal=Molecular Biotechnology |year=2000 |volume=16 |issue=1 |pages=23–52 |pmid= 11098467 |doi=10.1385/MB:16:1:23|s2cid=35874864 |doi-access=free }}</ref> Examples of yeast expression vector in ''Pichia'' are the pPIC series of vectors, and these vectors use the [[AOX1]] promoter which is inducible with [[methanol]].<ref>{{cite web |url=http://tools.invitrogen.com/content/sfs/brochures/B-067202_Pichia_Flyer.pdf |title=Pichia pastoris Expression System |work=Invitrogen }}</ref> The plasmids may contain elements for insertion of foreign DNA into the yeast genome and signal sequence for the secretion of expressed protein. Proteins with disulphide bonds and glycosylation can be efficiently produced in yeast. Another yeast used for protein production is ''[[Kluyveromyces lactis]]'' and the gene is expressed, driven by a variant of the strong [[lactase]] LAC4 promoter.<ref>{{cite web |url=https://www.neb.com/~/media/Catalog/All-Products/B1A99D5EBC6E45B3B876E40A8ECCED3F/Datacards%20or%20Manuals/manualE1000.pdf |title=K. lactis Protein Expression Kit |work=New England BioLabs Inc. |access-date=2013-03-20 |archive-date=2016-03-04 |archive-url=https://web.archive.org/web/20160304185904/https://www.neb.com/~/media/Catalog/All-Products/B1A99D5EBC6E45B3B876E40A8ECCED3F/Datacards%20or%20Manuals/manualE1000.pdf |url-status=dead }}</ref>

''[[Saccharomyces cerevisiae]]'' is particularly widely used for gene expression studies in yeast, for example in [[yeast two-hybrid system]] for the study of protein-protein interaction.<ref>{{cite journal |vauthors=Fields S, Song O |title=A novel genetic system to detect protein-protein interactions |journal=Nature |volume=340 |issue=6230 |pages=245–6 |year=1989 |pmid=2547163 |doi=10.1038/340245a0 |bibcode=1989Natur.340..245F |s2cid=4320733 }}</ref> The vectors used in yeast two-hybrid system contain fusion partners for two cloned genes that allow the transcription of a reporter gene when there is interaction between the two proteins expressed from the cloned genes.