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Particle-induced X-ray emission
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== Limitations == In order to get a meaningful sulfur signal from the analysis, the buffer should not contain sulfur (i.e. no BES, [[DDT]], [[HEPES]], [[MES (buffer)|MES]], [[MOPS]]O or [[PIPES]] compounds). Excessive amounts of [[chlorine]] in the buffer should also be avoided, since this will overlap with the sulfur peak; [[Potassium bromide|KBr]] and [[Sodium bromide|NaBr]] are suitable alternatives. Due to the low penetration depth of protons and heavy charged particles, PIXE is limited to analyzing the top micrometer of a given sample.
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