Editing Polysome (section)

== Profiling ==
[[Polysome profiling|Polysomal profiling]] is a technique that uses cycloheximide to arrest translation and a sucrose gradient to separate the resulting cell extract by centrifugation.<ref name=":1" /> Ribosome-associated mRNAs migrate faster than free mRNAs and polysome associated mRNAs migrate faster than ribosome associated mRNAs. Several peaks corresponding to mRNA are revealed by the measurement of total protein across the gradient. The corresponding mRNA is associated with increasing numbers of ribosomes as polysomes. The presence of mRNA across the gradient reveals the translation of the mRNA. Polysomal profiling is optimally applied to cultured cells and tissues to track the translational status of an identified mRNA as well as measure ribosome density.<ref name=":2" /> This technique has been used to compare the translational status of mRNAs in different cell types.

For example, polysomal profiling was used in a study to investigate the effect of vesicular stomatitis virus (VSV) in mammalian cells.<ref name=":3">{{cite journal | vauthors = Neidermyer WJ, Whelan SP | title = Global analysis of polysome-associated mRNA in vesicular stomatitis virus infected cells | journal = PLOS Pathogens | volume = 15 | issue = 6 | pages = e1007875 | date = June 2019 | pmid = 31226162 | pmc = 6608984 | doi = 10.1371/journal.ppat.1007875 | doi-access = free }}</ref> The data from polysomal profiling showed that host mRNAs are outcompeted by viral mRNAs for polysomes, therefore decreasing the translation of host mRNA and increasing the translation of viral mRNA.<ref name=":3" />