Editing GC-content (section)

=== Molecular biology ===
In [[polymerase chain reaction]] (PCR) experiments, the GC-content of short oligonucleotides known as [[primer (molecular biology)|primers]] is often used to predict their [[polymerase chain reaction|annealing temperature]] to the template DNA. A higher GC-content level indicates a relatively higher melting temperature.

Many sequencing technologies, such as [[Illumina sequencing]], have trouble reading high-GC-content sequences. [[Bird]] genomes are known to have many such parts, causing the problem of "missing genes" expected to be present from evolution and phenotype but never sequenced — until improved methods were used.<ref>{{cite journal|vauthors=Huttener R, Thorrez L, Veld TI |display-authors=etal|title=Sequencing refractory regions in bird genomes are hotspots for accelerated protein evolution|journal=BMC Ecol Evol|volume=21|issue=176|year=2021|page=176 |doi=10.1186/s12862-021-01905-7|pmid=34537008 |pmc=8449477 |doi-access=free}}</ref>