Editing Interactome (section)

===Validation===
First, the coverage and quality of an interactome has to be evaluated. Interactomes are never complete, given the limitations of experimental methods. For instance, it has been estimated that typical [[Yeast two-hybrid|Y2H]] screens detect only 25% or so of all interactions in an interactome.<ref name="Chen" /> The coverage of an interactome can be assessed by comparing it to benchmarks of well-known interactions that have been found and validated by independent assays.<ref name="Raja2009">{{Cite journal | last1 = Rajagopala | first1 = S. V. | last2 = Hughes | first2 = K. T. | last3 = Uetz | first3 = P. | doi = 10.1002/pmic.200900282 | title = Benchmarking yeast two-hybrid systems using the interactions of bacterial motility proteins | journal = Proteomics | volume = 9 | issue = 23 | pages = 5296–5302 | year = 2009 | pmid = 19834901 | pmc =2818629 }}</ref> Other methods filter out false positives calculating the similarity of known annotations of the proteins involved or define a likelihood of interaction using the subcellular localization of these proteins.<ref>{{Cite journal | author = [[Yanay Ofran]], [[Guy Yachdav]], [[Eyal Mozes]], [[Ta-tsen Soong]], [[Rajesh Nair]] & [[Burkhard Rost]] | title = Create and assess protein networks through molecular characteristics of individual proteins | journal = [[Bioinformatics (journal)|Bioinformatics]] | volume = 22 | issue = 14 | pages = e402–e407 | date = July 2006 | doi = 10.1093/bioinformatics/btl258 | pmid = 16873500 | doi-access = free }}</ref>