Open main menu
Home
Random
Recent changes
Special pages
Community portal
Preferences
About Wikipedia
Disclaimers
Incubator escapee wiki
Search
User menu
Talk
Dark mode
Contributions
Create account
Log in
Editing
Post-translational modification
(section)
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
===Non-enzymatic additions ''in vitro''=== * [[biotinylation]]: covalent attachment of a biotin moiety using a biotinylation reagent, typically for the purpose of labeling a protein. * carbamylation: the addition of isocyanic acid to a protein's N-terminus or the side-chain of Lys or Cys residues, typically resulting from exposure to urea solutions.<ref name="stark_1960">{{cite journal |vauthors=Stark GR, Stein WH, Moore X | title = Reactions of the Cyanate Present in Aqueous Urea with Amino Acids and Proteins | journal = J Biol Chem | volume = 235 | issue = 11 | pages = 3177β3181 | year = 1960 | doi = 10.1016/S0021-9258(20)81332-5 | doi-access = free }}</ref> * oxidation: addition of one or more oxygen atoms to a susceptible side-chain, principally of Met, Trp, His or Cys residues. Formation of [[disulfide]] bonds between Cys residues. * [[pegylation]]: covalent attachment of [[polyethylene glycol]] (PEG) using a pegylation reagent, typically to the N-terminus or the side-chains of Lys residues. Pegylation is used to improve the efficacy of protein pharmaceuticals.
Edit summary
(Briefly describe your changes)
By publishing changes, you agree to the
Terms of Use
, and you irrevocably agree to release your contribution under the
CC BY-SA 4.0 License
and the
GFDL
. You agree that a hyperlink or URL is sufficient attribution under the Creative Commons license.
Cancel
Editing help
(opens in new window)