Editing Affinity chromatography (section)

=== Specialty ===
Another use for affinity chromatography is the purification of specific proteins using a gel matrix that is unique to a specific protein. For example, the purification of E. coli β-galactosidase is accomplished by affinity chromatography using p-aminobenyl-1-thio-β-D-galactopyranosyl agarose as the affinity matrix. p-aminobenyl-1-thio-β-D-galactopyranosyl agarose is used as the affinity matrix because it contains a galactopyranosyl group, which serves as a good substrate analog for E. coli β-Galactosidase. This property allows the enzyme to bind to the stationary phase of the affinity matrix and β-Galactosidase is eluted by adding increasing concentrations of salt to the column.<ref>{{cite book|title=Fundamental Laboratory Approaches for Biochemistry and Biotechnology|last1=Ninfa|first1=Alexander J.|last2=Ballou|first2=David P.|last3=Benore|first3=Marilee|year=2006|edition=2nd|publisher=Wiley|page=153}}</ref>

==== Alkaline phosphatase ====
[[Alkaline phosphatase]] from E. coli can be purified using a DEAE-Cellulose matrix. A. phosphatase has a slight negative charge, allowing it to weakly bind to the positively charged amine groups in the matrix. The enzyme can then be eluted out by adding buffer with higher salt concentrations.<ref>{{Cite book|title=Fundamental laboratory approaches for biochemistry and biotechnology|last1=Ninfa|first1=Alexander J.|last2=Ballou|first2=David P.|last3=Benore|first3=Marilee|date=2010|publisher=John Wiley|isbn=9780470087664|edition=2nd|location=Hoboken, N.J.|page=240|oclc=420027217}}</ref>

==== Boronate affinity chromatography ====
Boronate affinity chromatography consists of using boronic acid or boronates to elute and quantify amounts of [[glycoprotein]]s. Clinical adaptations have applied this type of chromatography for use in determining long term assessment of diabetic patients through [[Glycated hemoglobin#Measurement|analysis of their glycated hemoglobin]].<ref name="Hage 1999" />