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===Detection method or technology=== Depending on the nature of the Detection system assays can be based on: #'''Colony forming''' or [[virtual colony count]]: e.g. by multiplying bacteria or proliferating cells. #'''[[Photometry (optics)|Photometry]]''' / '''[[spectrophotometry]]''' When the absorbance of a specific wavelength of light while passing through a fixed path-length through a cuvette of liquid test sample is measured and the absorbance is compared with a blank and standards with graded amounts of the target compound. If the emitted light is of a specific visible wavelength it may be called '''[[colorimetry]]''', or it may involve specific wavelength of light e.g. by use of [[laser]] and emission of [[fluorescent]] signals of another specific wavelength which is detected via very specific wavelength optical filters. #'''[[Transmittance]]''' of light may be used to measure e.g. clearing of opacity of a liquid created by suspended particles due to decrease in number of clumps during a platelet [[Agglutination (biology)|agglutination]] reaction. #'''[[Turbidimetry]]''' when the opacity of straight-transmitted light passing through a liquid sample is measured by detectors placed straight across the light source. #'''[[Nephelometry]]''' where a measurement of the amount of light scattering that occurs when a beam of light is passed through the solution is used to determine size and/or concentration and/or size distribution of particles in the sample.<ref>{{cite web |url= http://medical-dictionary.thefreedictionary.com/nephelometry|title=Nephelometry |date=2016 |website=The Free Dictionary |publisher=Farlex |access-date=9 September 2016 }}</ref> #'''[[Reflectometry]]''' When color of light reflected from a (usually dry) sample or reactant is assessed e.g. the automated readings of the strip urine dipstick assays. #Viscoelastic measurements e.g. viscometry, elastography (e.g. [[thromboelastography]]) #Counting assays: e.g. optic [[Flow cytometry|Flow cytometric]] cell or particle counters, or [[Coulter principle|coulter]]/impedance principle based cell counters #Imaging assays, that involve image analysis manually or by software: ##'''[[Cytometry]]''': When the size statistics of cells is assessed by an image processor. #Electric detection e.g. involving [[amperometry]], [[Voltammetry]], [[coulometry]] may be used directly or indirectly for many types of quantitative measurements. #Other physical property based assays may use ##[[Osmometer]] ##[[Viscometer]] ##[[Ion Selective electrode]]s ##[[Syndromic testing]]
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