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Microtome
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===Vibrating === The vibrating microtome operates by cutting using a vibrating blade, allowing the resultant cut to be made with less pressure than would be required for a stationary blade. The vibrating microtome is usually used for difficult biological samples.<ref name="Lang" /> The cut thickness is usually around 30β500 ΞΌm for live tissue and 10β500 ΞΌm for fixed tissue.<ref>{{Cite journal|last=Krumdieck|first=Carlos L.|date=January 2013|title=Development of a live tissue microtome: reflections of an amateur machinist|url=http://www.tandfonline.com/doi/full/10.3109/00498254.2012.724727|journal=Xenobiotica|language=en|volume=43|issue=1|pages=2β7|doi=10.3109/00498254.2012.724727|pmid=23009272|s2cid=6108637|issn=0049-8254|url-access=subscription}}</ref>
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