Editing Axoplasm

{{short description|Cytoplasm found within the axon of a neuron}}
{{Infobox anatomy
| Name        = Axoplasm
| Latin       = axoplasma
| Image       =
| Caption     =
| Image2      =
| Caption2    =
| Precursor   =
| System      = [[Nervous system]]
| PartOf = [[Axon]] of a [[nerve]]
}}
'''Axoplasm''' is the [[cytoplasm]] within the [[axon]] of a [[neuron]] (nerve cell). For some neuronal types this can be more than 99% of the total cytoplasm.<ref>{{cite journal |last1=Sabry |first1=J. |last2=O’Connor |first2=T. P. |last3=Kirschner |first3=M. W. |year=1995 |title=Axonal Transport of Tubulin in Ti1 Pioneer Neurons in Situ |journal=Neuron |volume=14 |issue=6 |pages=1247–1256 |doi=10.1016/0896-6273(95)90271-6 |pmid=7541635 |doi-access=free }}</ref>

Axoplasm has a different composition of [[organelle]]s and other materials than that found in the neuron's [[cell (biology)|cell]] body ([[soma (cell body)|soma]]) or dendrites. In [[axonal transport]] (also known as axoplasmic transport) materials are carried through the axoplasm to or from the soma.

The [[electrical resistance]] of the axoplasm, called axoplasmic resistance, is one aspect of a neuron's cable properties, because it affects the rate of travel of an [[action potential]] down an axon. If the axoplasm contains many [[molecule]]s that are not [[electrical conduction|electrically conductive]], it will slow the travel of the potential because it will cause more [[ion]]s to flow across the [[axolemma]] (the axon's membrane) than through the axoplasm.

==Structure==
Axoplasm is composed of various organelles and cytoskeletal elements. The axoplasm contains a high concentration of elongated [[mitochondria]], [[microfilament]]s, and [[microtubules]].<ref>{{cite book |last=Hammond |first=C. |year=2015 |title =Cellular and Molecular Neurophysiology |pages=433 |publisher=  Academic Press |asin=B00XV3J0UE |edition=4th }}</ref> Axoplasm lacks much of the cellular machinery ([[ribosomes]] and [[Cell nucleus|nucleus]]) required to [[Transcription (genetics)|transcribe]] and translate complex [[proteins]]. As a result, most enzymes and large proteins are transported from the soma through the axoplasm. Axonal transport occurs either by fast or slow transport. Fast transport involves vesicular contents (like organelles) being moved along microtubules by [[motor proteins]] at a rate of 50–400mm per day.<ref>{{cite book |last=Brady |first=S. T. |year=1993 |title=Axonal dynamics and regeneration |publisher=New York: Raven Press |pages=7–36 }}</ref> Slow axoplasmic transport involves the movement of cytosolic soluble proteins and cytoskeletal elements at a much slower rate of 0.02-0.1mm/d. The precise mechanism of slow axonal transport remains unknown but recent studies have proposed that it may function by means of transient association with the fast axonal transport [[vesicle (biology and chemistry)|vesicle]]s.<ref>{{cite journal |last=Young |first=Tang |year=2013 |title=Fast Vesicle Transport Is Required for the Slow Axonal Transport of Synapsin. |journal=Neuroscience |volume=33  |issue=39 |pages=15362–15375 |doi=10.1523/jneurosci.1148-13.2013|pmid=24068803 |pmc=3782618 }}</ref> Though axonal transport is responsible for most organelles and complex proteins present in the axoplasm, recent studies have shown that some translation does occur in axoplasm. This axoplasmic translation is possible due to the presence of localized translationally silent [[mRNA]] and ribonuclear [[protein complexes]].<ref name=Piper>{{cite journal |last1=Piper |first1=M |last2=Holt|first2= C. |year=2004 |title=RNA Translation in Axons. |journal=Annual Review of Cell and Developmental Biology |volume=20  |pages=505–523 |doi=10.1146/annurev.cellbio.20.010403.111746|pmid=15473850 |pmc=3682640}}</ref>

==Function==

=== Signal transduction ===
{{Unreferenced|section|date=February 2020}}
Axoplasm is integral to the overall function of neurons in propagating action potential through the axon. The amount of axoplasm in the axon is important to the cable like properties of the axon in cable theory. In regards to [[cable theory]], the axoplasmic content determines the resistance of the axon to a potential change. The composing cytoskeletal elements of axoplasm, neural filaments, and microtubules provide the framework for axonal transport which allows for [[neurotransmitters]] to reach the [[synapse]]. Furthermore, axoplasm contains the pre-synaptic vesicles of neurotransmitter which are eventually released into the [[synaptic cleft]].

=== Damage detection and regeneration ===
Axoplasm contains both the mRNA and ribonuclearprotein required for axonal protein synthesis. Axonal protein synthesis has been shown to be integral in both [[neural regeneration]] and in localized responses to axon damage.<ref name=Piper/> When an axon is damaged, both axonal translation and retrograde axonal transport are required to propagate a signal to the soma that the cell is damaged.<ref name=Piper/>

==History==

Axoplasm was not a main focus for neurological research until after many years of learning of the functions and properties of [[squid giant axon]]s. Axons in general were very difficult to study due to their narrow structure and in close proximity to [[glial cells]].<ref>{{cite journal |last=Gilbert |first=D. |year=1975 |title=Axoplasm chemical composition in Myxicola and solubility properties of its structural proteins |journal=The Journal of Physiology |volume=253 |issue=1 |pages=303–319 |doi=10.1113/jphysiol.1975.sp011191|pmid=1260 |pmc=1348544 }}</ref> To solve this problem squid axons were used as an animal model due to the relatively vast sized axons compared to humans or other mammals.<ref>{{cite book |last=Young |first=J. |year=1977 |title=What squids and octopuses tell us about brains and memories. |publisher= American Museum of Natural History. |edition=1}}</ref> These axons were mainly studied to understand action potential, and axoplasm was soon understood to be important in [[membrane potential]].<ref>{{cite journal |last1=Steinbach |first1=H. |last2=Spiegelman|first2=S. |year=1943 |title=The sodium and potassium balance in squid nerve axoplasm |journal= Journal of Cellular and Comparative Physiology|volume=22 |issue=2 |pages=187–196 |doi=10.1002/jcp.1030220209}}</ref> The axoplasm was at first just thought to be very similar to cytoplasm, but axoplasm plays an important role in transference of nutrients and electrical potential that is generated by neurons.<ref>{{cite journal |last=Bloom |first=G. |year=1993 |title=GTP gamma S inhibits organelle transport along axonal microtubules. |journal=  The Journal of Cell Biology|volume=120 |issue=2 |pages=467–476 |doi=10.1083/jcb.120.2.467|pmid=7678421 |pmc=2119514 }}</ref>

It actually proves quite difficult to isolate axons from the [[myelin]] that surrounds it,<ref>{{cite journal |last1=DeVries |first1=G. |last2=Norton|first2=W. |last3=Raine|first3=C.| year=1972 |title=Axons: isolation from mammalian central nervous system. |journal=Science |volume=175 |issue=4028 |pages=1370–1372 |doi=10.1126/science.175.4028.1370|pmid=4551023 |bibcode=1972Sci...175.1370D |s2cid=30934150 }}</ref> so the squid giant axon is the focus for many studies that touch on axoplasm. As more knowledge formed from studying the signalling that occurs in neurons, transfer of nutrients and materials became an important topic to research. The mechanisms for the proliferation and sustained electrical potentials were affected by the fast axonal transport system. The fast axonal transport system uses the axoplasm for movement, and contains many non-conductive molecules that change the rate of these electrical potentials across the axon,<ref>{{cite journal |last=Brady |first=S.| year=1985 |title=A novel brain ATPase with properties expected for the fast axonal transport motor. |journal=Nature |volume=317 |issue=6032|pages=73–75 |doi=10.1038/317073a0|pmid=2412134|bibcode=1985Natur.317...73B|s2cid=4327023}}</ref> but the opposite influence does not occur. The fast axonal transport system is able to function without an axolemma, implying that the electrical potential does not influence the transport of materials through the axon.<ref>{{cite journal |last1=Brady |first1=S.| last2=Lasek|first2=R. |last3=Allen|first3=R.|year=1982 |title=Fast axonal transport in extruded axoplasm from squid giant axon. |journal=Science |volume=218 |issue=4577|pages=1129–1131 |doi=10.1126/science.6183745|pmid=6183745|bibcode=1982Sci...218.1129B}}</ref> This understanding of the relationship of axoplasm regarding transport and electrical potential is critical in the understanding of the overall brain functions.

With this knowledge, axoplasm has become a model for studying varying cell signaling and functions for the research of neurological diseases like [[Alzheimer's]],<ref>{{cite journal |last1=Kanaan |first1=N.| last2=Morfini|first2=G. |last3=LaPointe|first3=N. |last4=Pigino|first4=G. |last5=Patterson|first5=K. |last6=Song|first6=Y.|last7=Andreadis |first7=A.|last8=Fu |first8=Y.|last9=Brady|first9=S.|last10=Binder|first10=L.|year=2011 |title=Pathogenic forms of tau inhibit kinesin-dependent axonal transport through a mechanism involving activation of axonal phosphotransferases. |journal=Neuroscience |volume=31 |issue=27|pages=9858–9868 |doi=10.1523/jneurosci.0560-11.2011|pmid=21734277|pmc=3391724}}</ref> and [[Huntington's]].<ref>{{cite journal| last1=Morfini|first1=G. |last2=You|first2=Y. |last3=Pollema |first3=S. |last4=Kaminska |first4=A. |last5=Liu |first5=K. |last6=Yoshioka |first6=K. |last7=Björkblom |first7=B. |last8=Coffey |first8=E. |last9=Bagnato |first9=C. |last10=Han |first10=D. |year=2009 |title= Pathogenic huntingtin inhibits fast axonal transport by activating JNK3 and phosphorylating kinesin. |journal= Nature Neuroscience |volume=12 |issue=7 |pages=864–871 |doi=10.1038/nn.2346|pmid=19525941 |pmc=2739046 }}</ref> Fast axonal transport is a crucial mechanism when examining these diseases and determining how a lack of materials and nutrients can influence the progression of neurological disorders.

==References==
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{{Nervous tissue}}
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[[Category:Neurohistology]]