Editing Model organism

{{short description|Organisms used to study biology across species}}
[[File:E coli at 10000x, original.jpg|thumb | right | ''[[Escherichia coli]]'' is a [[gram-negative bacteria|gram-negative]] [[prokaryote|prokaryotic]] model organism]]
[[File:Drosophila melanogaster - side (aka).jpg|thumb | ''[[Drosophila melanogaster]]'', one of the most famous subjects for [[genetics]] experiments]]
[[File:S cerevisiae under DIC microscopy.jpg|thumb | ''[[Saccharomyces cerevisiae]]'', one of the most intensively studied [[Eukaryote|eukaryotic]] model organisms in [[molecular biology|molecular]] and [[cell biology]]]]

{{also|List of model organisms}}
A '''model organism''' is a [[non-human]] [[species]] that is extensively studied to understand particular [[biology|biological]] phenomena, with the expectation that discoveries made in the [[model]] organism will provide insight into the workings of other organisms.<ref>{{cite journal |last1=Fields |first1=S. |last2=Johnston |first2=M |title=CELL BIOLOGY: Whither Model Organism Research? |journal=Science |date=2005-03-25 |volume=307 |issue=5717 |pages=1885–1886 |doi=10.1126/science.1108872 |pmid=15790833 }}</ref><ref>Griffiths, E. C. (2010) [http://www.emily-griffiths.postgrad.shef.ac.uk/models.pdf What is a model?] {{webarchive |url=https://web.archive.org/web/20120312220527/http://www.emily-griffiths.postgrad.shef.ac.uk/models.pdf |date=March 12, 2012 }}</ref> Model organisms are widely used to research human [[disease]] when [[human experimentation]] would be unfeasible or [[bioethics|unethical]].<ref>{{cite book|url=https://books.google.com/books?id=yTfNH3cScKAC<!--confirmed ISBN match; full text access-->|title=The Case for Animal Experimention: An Evolutionary and Ethical Perspective|last=Fox|first=Michael Allen|publisher=University of California Press|year=1986|isbn=978-0-520-05501-8|location=Berkeley and Los Angeles, California|oclc=11754940|via=Google Books}}</ref> This strategy is made possible by the [[common descent]] of all living organisms, and the conservation of [[Metabolic pathway|metabolic]] and [[developmental biology|developmental]] pathways and [[genetic material]] over the course of [[evolution]].<ref>{{cite journal |last1=Allmon |first1=Warren D. |last2=Ross |first2=Robert M. |title=Evolutionary remnants as widely accessible evidence for evolution: the structure of the argument for application to evolution education |journal=Evolution: Education and Outreach |date=December 2018 |volume=11 |issue=1 |pages=1 |doi=10.1186/s12052-017-0075-1 |doi-access=free }}</ref>

Research using animal models has been central to most of the achievements of modern medicine.<ref name=RSM2015/><ref name=NRCIOM/><ref name="Nature2007"/> It has contributed most of the basic knowledge in fields such as human [[physiology]] and [[biochemistry]], and has played significant roles in fields such as [[neuroscience]] and [[infectious disease]].<ref name=NRCIOMb/><ref name="HLAS2011"/> The results have included the near-[[Poliomyelitis eradication|eradication of polio]] and the development of [[organ transplantation]], and have benefited both humans and animals.<ref name=RSM2015/><ref name="IOM1991"/> From 1910 to 1927, [[Thomas Hunt Morgan]]'s work with the fruit fly ''[[Drosophila melanogaster]]'' identified [[chromosome]]s as the vector of inheritance for genes,<ref name="nobelprize.org"/><ref name="nobel2"/> and [[Eric Kandel]] wrote that Morgan's discoveries "helped transform biology into an experimental science".<ref name="Kandel1999"/> Research in model organisms led to further medical advances, such as the production of the [[diphtheria antitoxin]]<ref name="nobel3"/><ref name="Cannon2009"/> and the 1922 discovery of [[insulin]]<ref name="insulin"/> and its use in treating diabetes, which had previously meant death.<ref name="Thompson2009"/> Modern general anaesthetics such as [[halothane]] were also developed through studies on model organisms, and are necessary for modern, complex surgical operations.<ref name="raventos1956"/> Other 20th-century medical advances and treatments that relied on research performed in animals include [[organ transplant]] techniques,<ref name="carrel1912"/><ref name="williamson1926">Williamson C (1926) ''J. Urol.'' 16: p. 231</ref><ref name="woodruff1986"/><ref name="moore1964"/> the heart-lung machine,<ref name="gibbon1937"/> [[antibiotic]]s,<ref name="rawbw"/><ref name="Streptomycin"/><ref name="fleming1929"/> and the [[whooping cough]] vaccine.<ref name="mrc1956"/>

In researching human [[disease]], model organisms allow for better understanding the disease process without the added risk of harming an actual human. The species of the model organism is usually chosen so that it reacts to disease or its treatment in a way that resembles human [[physiology]], even though care must be taken when generalizing from one organism to another.<ref>{{Cite book|title=Essential Developmental Biology|last=Slack|first=Jonathan M. W.|publisher=Wiley-Blackwell|year=2013|location=Oxford|oclc=785558800}}</ref> However, many drugs, treatments and cures for human diseases are developed in part with the guidance of animal models.<ref name="zam">{{cite journal |last1=Chakraborty |first1=Chiranjib |last2=Hsu |first2=Chi |last3=Wen |first3=Zhi |last4=Lin |first4=Chang |last5=Agoramoorthy |first5=Govindasamy |title=Zebrafish: A Complete Animal Model for In Vivo Drug Discovery and Development |journal=Current Drug Metabolism |date=2009-02-01 |volume=10 |issue=2 |pages=116–124 |doi=10.2174/138920009787522197 |pmid=19275547 }}</ref><ref name=zrug>{{cite journal |last1=Kari |first1=G |last2=Rodeck |first2=U |last3=Dicker |first3=A P |title=Zebrafish: An Emerging Model System for Human Disease and Drug Discovery |journal=Clinical Pharmacology & Therapeutics |date=July 2007 |volume=82 |issue=1 |pages=70–80 |doi=10.1038/sj.clpt.6100223 |pmid=17495877 }}</ref> Treatments for animal diseases have also been developed, including for [[rabies]],<ref name="buck1904"/> [[anthrax]],<ref name="buck1904" /> [[glanders]],<ref name="buck1904" /> [[feline immunodeficiency virus]] (FIV),<ref name="pu2005"/> [[tuberculosis]],<ref name="buck1904" /> Texas cattle fever,<ref name="buck1904" /> [[classical swine fever]] (hog cholera),<ref name="buck1904" /> [[heartworm]], and other [[Parasitic disease|parasitic infections]].<ref name="dryden2005"/> Animal experimentation continues to be required for biomedical research,<ref name=bundle/> and is used with the aim of solving medical problems such as Alzheimer's disease,<ref name="geula1998"/> AIDS,<ref name="AIDS2005"/> multiple sclerosis,<ref name="jameson1994"/> spinal cord injury, many headaches,<ref name="lyuksyutova1984"/> and other conditions in which there is no useful ''[[in vitro]]'' model system available.

Model organisms are drawn from all three [[Domain (biology)|domains]] of life, as well as [[virus]]es. One of the first model systems for [[molecular biology]] was the bacterium ''[[Escherichia coli]]'' (''E. coli''), a common constituent of the human digestive system. The mouse (''[[House mouse|Mus musculus]]'') has been used extensively as a model organism and is associated with many important biological discoveries of the 20th and 21st centuries.<ref name="Hedrich"/> Other examples include baker's yeast (''[[Saccharomyces cerevisiae]]''), the [[T4 phage]] virus, the [[Drosophilidae|fruit fly]] ''[[Drosophila melanogaster]]'', the flowering plant ''[[Arabidopsis thaliana]]'', and [[guinea pig]]s (''Cavia porcellus''). Several of the bacterial viruses ([[bacteriophage]]) that infect ''[[Escherichia coli|E. coli]]'' also have been very useful for the study of gene structure and [[gene regulation]] (e.g. phages [[Lambda phage|Lambda]] and [[Enterobacteria phage T4|T4]]).<ref>{{cite journal |last1=Grada |first1=Ayman |last2=Mervis |first2=Joshua |last3=Falanga |first3=Vincent |date=October 2018 |title=Research Techniques Made Simple: Animal Models of Wound Healing |journal=Journal of Investigative Dermatology |volume=138 |issue=10 |pages=2095–2105.e1 |doi=10.1016/j.jid.2018.08.005 |pmid=30244718 |doi-access=free}}</ref> Disease models are divided into three categories: homologous animals have the same causes, symptoms and treatment options as would humans who have the same disease, isomorphic animals share the same symptoms and treatments, and predictive models are similar to a particular human disease in only a couple of aspects, but are useful in isolating and making predictions about mechanisms of a set of disease features.<ref>{{cite web | url=http://academic.uprm.edu/~ephoebus/id85.htm | title=Pinel Chapter 6 - Human Brain Damage & Animal Models | publisher=Academic.uprm.edu | access-date=2014-01-10 | archive-url=https://web.archive.org/web/20141013041340/http://academic.uprm.edu/~ephoebus/id85.htm | archive-date=2014-10-13 | url-status=dead }}</ref>

==History==
The use of animals in research dates back to [[ancient Greece]], with [[Aristotle]] (384–322 BCE) and [[Erasistratus]] (304–258 BCE) among the first to perform experiments on living animals.<ref>Cohen BJ, Loew FM. (1984) Laboratory Animal Medicine: Historical Perspectives in Laboratory Animal Medicine Academic Press, Inc: Orlando, FL, USA; Fox JG, Cohen BJ, Loew FM (eds)</ref> Discoveries in the 18th and 19th centuries included [[Antoine Lavoisier]]'s use of a [[guinea pig]] in a [[calorimeter]] to prove that [[Respiration (physiology)|respiration]] was a form of combustion, and [[Louis Pasteur]]'s demonstration of the [[germ theory of disease]] in the 1880s using [[anthrax]] in sheep.<ref name="pmid11544370">{{cite journal | vauthors = Mock M, Fouet A | title = Anthrax | journal = Annu. Rev. Microbiol. | volume = 55 | pages = 647–71 | year = 2001 | pmid = 11544370 | doi = 10.1146/annurev.micro.55.1.647 }}</ref>

Research using animal models has been central to most of the achievements of modern medicine.<ref name=RSM2015>{{cite web| title = Statement of the Royal Society's position on the use of animals in research| author = Royal Society of Medicine| date = 13 May 2015| url = https://royalsociety.org/topics-policy/publications/2015/animals-in-research/|quote=From antibiotics and insulin to blood transfusions and treatments for cancer or HIV, virtually every medical achievement in the past century has depended directly or indirectly on research using animals, including veterinary medicine.}}</ref><ref name=NRCIOM>{{cite book|author=[[National Research Council (United States)|National Research Council]] and [[Institute of Medicine]]|title=Use of Laboratory Animals in Biomedical and Behavioral Research|url=https://books.google.com/books?id=EzorAAAAYAAJ|date=1988|publisher=National Academies Press|page=37|isbn=9780309038393|id=NAP:13195|quote=The...methods of scientific inquiry have greatly reduced the incidence of human disease and have substantially increased life expectancy. Those results have come largely through experimental methods based in part on the use of animals.}}</ref><ref name="Nature2007">{{cite journal |last1=Lieschke |first1=Graham J. |last2=Currie |first2=Peter D. |title=Animal models of human disease: zebrafish swim into view |journal=Nature Reviews Genetics |date=May 2007 |volume=8 |issue=5 |pages=353–367 |doi=10.1038/nrg2091 |pmid=17440532 |quote=Biomedical research depends on the use of animal models to understand the pathogenesis of human disease at a cellular and molecular level and to provide systems for developing and testing new therapies. }}</ref> It has contributed most of the basic knowledge in fields such as human [[physiology]] and [[biochemistry]], and has played significant roles in fields such as [[neuroscience]] and [[infectious disease]].<ref name=NRCIOMb>{{cite book|author=[[National Research Council (United States)|National Research Council]] and [[Institute of Medicine]]|title=Use of Laboratory Animals in Biomedical and Behavioral Research|url=https://books.google.com/books?id=EzorAAAAYAAJ|date=1988|publisher=National Academies Press|page=27|isbn=9780309038393|id=NAP:13195|quote=Animal studies have been an essential component of every field of medical research and have been crucial for the acquisition of basic knowledge in biology.}}</ref><ref name="HLAS2011">Hau and Shapiro 2011:
* {{cite book|author1=Jann Hau|author2=Steven J. Schapiro|title=Handbook of Laboratory Animal Science, Volume I, Third Edition: Essential Principles and Practices|url=https://books.google.com/books?id=D-IHAaggi_4C|year=2011|publisher=CRC Press|page=2|isbn=978-1-4200-8456-6|quote=Animal-based research has played a key role in understanding infectious diseases, neuroscience, physiology, and toxicology. Experimental results from animal studies have served as the basis for many key biomedical breakthroughs.}}
* {{cite book|author1=Jann Hau|author2=Steven J. Schapiro|title=Handbook of Laboratory Animal Science, Volume II, Third Edition: Animal Models|url=https://books.google.com/books?id=yk7TFvsFCBcC|year=2011|publisher=CRC Press|page=1|isbn=978-1-4200-8458-0|quote=Most of our basic knowledge of human biochemistry, physiology, endocrinology, and pharmacology has been derived from initial studies of mechanisms in animal models.}}</ref> For example, the results have included the near-[[Poliomyelitis eradication|eradication of polio]] and the development of [[organ transplantation]], and have benefited both humans and animals.<ref name=RSM2015/><ref name="IOM1991">{{cite book|author=Institute of Medicine|title=Science, Medicine, and Animals|url=https://archive.org/details/sciencemedicinea00comm|url-access=registration|date=1991|publisher=National Academies Press|isbn=978-0-309-56994-1|page=[https://archive.org/details/sciencemedicinea00comm/page/3 3]|quote=...without this fundamental knowledge, most of the clinical advances described in these pages would not have occurred.}}</ref> From 1910 to 1927, [[Thomas Hunt Morgan]]'s work with the fruit fly ''[[Drosophila melanogaster]]'' identified [[chromosome]]s as the vector of inheritance for genes.<ref name="nobelprize.org">{{cite web|title=The Nobel Prize in Physiology or Medicine 1933|url=http://nobelprize.org/nobel_prizes/medicine/laureates/1933/index.html|access-date=2015-06-20|publisher=Nobel Web AB}}</ref><ref name="nobel2">{{cite web|title=Thomas Hunt Morgan and his Legacy|url=https://www.nobelprize.org/nobel_prizes/medicine/laureates/1933/morgan-article.html|access-date=2015-06-20|publisher=Nobel Web AB}}</ref> ''Drosophila'' became one of the first, and for some time the most widely used, model organisms,<ref>Kohler, ''Lords of the Fly'', chapter 5</ref> and [[Eric Kandel]] wrote that Morgan's discoveries "helped transform biology into an experimental science".<ref name="Kandel1999">Kandel, Eric. 1999. [http://www.columbia.edu/cu/alumni/Magazine/Legacies/Morgan/ "Genes, Chromosomes, and the Origins of Modern Biology"], ''Columbia Magazine''</ref> ''D. melanogaster'' remains one of the most widely used eukaryotic model organisms. During the same time period, studies on mouse genetics in the laboratory of [[William Ernest Castle]] in collaboration with [[Abbie Lathrop]] led to generation of the DBA ("dilute, brown and non-agouti") inbred mouse strain and the systematic generation of other inbred strains.<ref name="Steensma">{{cite journal|last=Steensma|first=David P. |author2=Kyle Robert A. |author3=Shampo Marc A.|date=November 2010|title=Abbie Lathrop, the "Mouse Woman of Granby": Rodent Fancier and Accidental Genetics Pioneer|journal=Mayo Clinic Proceedings|volume=85|issue=11|pmc=2966381|pmid=21061734|doi=10.4065/mcp.2010.0647|pages=e83}}</ref><ref>{{cite web|url=https://immunology.hms.harvard.edu/about-us/history|title=History of Immunology at Harvard|last=Pillai|first=Shiv|work=Harvard Medical School:About us|publisher=Harvard Medical School|access-date=19 December 2013|archive-url=https://web.archive.org/web/20131220022416/https://immunology.hms.harvard.edu/about-us/history|archive-date=20 December 2013|url-status=dead}}</ref> The mouse has since been used extensively as a model organism and is associated with many important biological discoveries of the 20th and 21st centuries.<ref name="Hedrich">{{cite book|title=The Laboratory Mouse|editor= Hedrich, Hans|publisher=Elsevier Science|chapter=The house mouse as a laboratory model: a historical perspective|isbn=9780080542539|date= 2004-08-21}}</ref>

In the late 19th century, [[Emil von Behring]] isolated the [[diphtheria]] toxin and demonstrated its effects in guinea pigs. He went on to develop an antitoxin against diphtheria in animals and then in humans, which resulted in the modern methods of immunization and largely ended diphtheria as a threatening disease.<ref name="nobel3">[http://nobelprize.org/nobel_prizes/medicine/laureates/1901/behring-bio.html Bering Nobel Biography]</ref> The diphtheria antitoxin is famously commemorated in the Iditarod race, which is modeled after the delivery of antitoxin in the [[1925 serum run to Nome]]. The success of animal studies in producing the diphtheria antitoxin has also been attributed as a cause for the decline of the early 20th-century opposition to animal research in the United States.<ref name="Cannon2009">[http://www.amphilsoc.org/library/mole/c/cannon.htm Walter B. Cannon Papers, American Philosophical Society] {{webarchive |url=https://web.archive.org/web/20090814184304/http://www.amphilsoc.org/library/mole/c/cannon.htm |date=August 14, 2009 }}</ref>

Subsequent research in model organisms led to further medical advances, such as [[Frederick Banting]]'s research in dogs, which determined that the isolates of pancreatic secretion could be used to treat dogs with [[Diabetes mellitus|diabetes]]. This led to the 1922 discovery of [[insulin]] (with [[John Macleod (physiologist)|John Macleod]])<ref name="insulin">[http://www.mta.ca/faculty/arts/canadian_studies/english/about/study_guide/doctors/insulin.html Discovery of Insulin] {{webarchive |url=https://web.archive.org/web/20090930142937/http://www.mta.ca/faculty/arts/canadian_studies/english/about/study_guide/doctors/insulin.html |date=September 30, 2009 }}</ref> and its use in treating diabetes, which had previously meant death.<ref name="Thompson2009">[http://www.dlife.com/dLife/do/ShowContent/inspiration_expert_advice/famous_people/leonard_thompson.html Thompson bio ref] {{webarchive|url=https://web.archive.org/web/20090210030429/http://www.dlife.com/dLife/do/ShowContent/inspiration_expert_advice/famous_people/leonard_thompson.html |date=2009-02-10 }}</ref> [[John Cade]]'s research in guinea pigs discovered the anticonvulsant properties of lithium salts,<ref>[http://www.adb.online.anu.edu.au/biogs/A130374b.htm] John Cade and Lithium</ref> which revolutionized the treatment of [[bipolar disorder]], replacing the previous treatments of lobotomy or electroconvulsive therapy. Modern general anaesthetics, such as [[halothane]] and related compounds, were also developed through studies on model organisms, and are necessary for modern, complex surgical operations.<ref name="raventos1956">Raventos J (1956) ''Br J Pharmacol'' 11, 394</ref><ref name="whalen2005">Whalen FX, Bacon DR & Smith HM (2005) ''Best Pract Res Clin Anaesthesiol'' 19, 323</ref>

In the 1940s, [[Jonas Salk]] used rhesus monkey studies to isolate the most virulent forms of the [[polio]] virus,<ref>{{cite web |url=http://www.post-gazette.com/pg/05093/481117.stm |title=Developing a medical milestone: The Salk polio vaccine |access-date=2015-06-20 |url-status=dead |archive-url=https://web.archive.org/web/20100311191427/http://www.post-gazette.com/pg/05093/481117.stm |archive-date=2010-03-11 }} Virus-typing of polio by Salk</ref> which led to his creation of a [[polio vaccine]]. The vaccine, which was made publicly available in 1955, reduced the incidence of polio 15-fold in the United States over the following five years.<ref>{{cite web |url=http://www.post-gazette.com/pg/05094/482468.stm |title=Tireless polio research effort bears fruit and indignation |access-date=2008-08-23 |url-status=dead |archive-url=https://web.archive.org/web/20080905022100/http://www.post-gazette.com/pg/05094/482468.stm |archive-date=2008-09-05 }} Salk polio virus</ref> [[Albert Sabin]] improved the vaccine by passing the polio virus through animal hosts, including monkeys; the Sabin vaccine was produced for mass consumption in 1963, and had virtually eradicated polio in the United States by 1965.<ref>[http://americanhistory.si.edu/polio/virusvaccine/vacraces2.htm] {{Webarchive|url=https://web.archive.org/web/20110604021151/http://americanhistory.si.edu/polio/virusvaccine/vacraces2.htm |date=2011-06-04 }} History of polio vaccine</ref> It has been estimated that developing and producing the vaccines required the use of 100,000 rhesus monkeys, with 65 doses of vaccine produced from each monkey. Sabin wrote in 1992, "Without the use of animals and human beings, it would have been impossible to acquire the important knowledge needed to prevent much suffering and premature death not only among humans, but also among animals."<ref>[http://www.animalresearch.info/en/resources/163/-the-work-on-polio-prevention-was-long-dela/ "the work on [polio&#93; prevention was long delayed by... misleading experimental models of the disease in monkeys" | ari.info<!-- Bot generated title -->]</ref>

Other 20th-century medical advances and treatments that relied on research performed in animals include [[organ transplant]] techniques,<ref name="carrel1912">Carrel A (1912) ''Surg. Gynec. Obst.'' 14: p. 246</ref><ref name="williamson1926">Williamson C (1926) ''J. Urol.'' 16: p. 231</ref><ref name="woodruff1986">Woodruff H & Burg R (1986) in ''Discoveries in Pharmacology'' vol 3, ed Parnham & Bruinvels, Elsevier, Amsterdam</ref><ref name="moore1964">Moore F (1964) ''Give and Take: the Development of Tissue Transplantation''. Saunders, New York</ref> the heart-lung machine,<ref name="gibbon1937">Gibbon JH (1937) ''Arch. Surg.'' 34, 1105</ref> [[antibiotic]]s,<ref name="rawbw">[http://www.rawbw.com/~hinshaw/cgi-bin/id?1375] Hinshaw obituary</ref><ref name="Streptomycin">[http://www.discoveriesinmedicine.com/Ra-Thy/Streptomycin.html] Streptomycin</ref><ref name="fleming1929">Fleming A (1929) ''Br J Exp Path'' 10, 226</ref> and the [[whooping cough]] vaccine.<ref name="mrc1956">Medical Research Council (1956) ''Br. Med. J.'' 2: p. 454</ref> Treatments for animal diseases have also been developed, including for [[rabies]],<ref name="buck1904">''A reference handbook of the medical sciences''. William Wood and Co., 1904, Edited by Albert H. Buck.</ref> [[anthrax]],<ref name="buck1904" /> [[glanders]],<ref name="buck1904" /> [[feline immunodeficiency virus]] (FIV),<ref name="pu2005">{{cite journal |last1=Pu |first1=Ruiyu |last2=Coleman |first2=James |last3=Coisman |first3=James |last4=Sato |first4=Eiji |last5=Tanabe |first5=Taishi |last6=Arai |first6=Maki |last7=Yamamoto |first7=Janet K |title=Dual-subtype FIV vaccine (Fel-O-Vax® FIV) protection against a heterologous subtype B FIV isolate |journal=Journal of Feline Medicine and Surgery |date=February 2005 |volume=7 |issue=1 |pages=65–70 |doi=10.1016/j.jfms.2004.08.005 |pmid=15686976 |pmc=10911555 }}</ref> [[tuberculosis]],<ref name="buck1904" /> Texas cattle fever,<ref name="buck1904" /> [[classical swine fever]] (hog cholera),<ref name="buck1904" /> [[heartworm]], and other [[Parasitic disease|parasitic infections]].<ref name="dryden2005">{{cite journal | last1 = Dryden | first1 = MW | last2 = Payne | first2 = PA | title = Preventing parasites in cats | journal = Veterinary Therapeutics | volume = 6 | issue = 3 | pages = 260–7 | year = 2005 | pmid = 16299672 }}</ref> Animal experimentation continues to be required for biomedical research,<ref name=bundle>Sources:
* {{cite book|author=P. Michael Conn|title=Animal Models for the Study of Human Disease|url=https://books.google.com/books?id=dVLVLIV8rD0C|date=29 May 2013|publisher=Academic Press|isbn=978-0-12-415912-9|page=37|quote=...animal models are central to the effective study and discovery of treatments for human diseases.}}
* {{cite journal |last1=Lieschke |first1=Graham J. |last2=Currie |first2=Peter D. |title=Animal models of human disease: zebrafish swim into view |journal=Nature Reviews Genetics |date=May 2007 |volume=8 |issue=5 |pages=353–367 |doi=10.1038/nrg2091 |pmid=17440532 |quote=Biomedical research depends on the use of animal models to understand the pathogenesis of human disease at a cellular and molecular level and to provide systems for developing and testing new therapies.}}
* {{cite book|author1=Pierce K. H. Chow|author2=Robert T. H. Ng|author3=Bryan E. Ogden|title=Using Animal Models in Biomedical Research: A Primer for the Investigator|url=https://books.google.com/books?id=NtWM8gD9Z2MC|year=2008|publisher=World Scientific|isbn=978-981-281-202-5|pages=1–2|quote=Arguments regarding whether biomedical science can advance without the use of animals are frequently mooted and make as much sense as questioning if clinical trials are necessary before new medical therapies are allowed to be widely used in the general population [pg. 1] ...animal models are likely to remain necessary until science develops alternative models and systems that are equally sound and robust [pg. 2].}}
* {{cite book|author1=Jann Hau|author2=Steven J. Schapiro|title=Handbook of Laboratory Animal Science, Volume I, Third Edition: Essential Principles and Practices|chapter-url=https://books.google.com/books?id=D-IHAaggi_4C|year=2011|publisher=CRC Press|chapter=The contribution of laboratory animals to medical progress|isbn=978-1-4200-8456-6|quote=Animal models are required to connect [modern biological technologies] in order to understand whole organisms, both in healthy and diseased states. In turn, these animal studies are required for understanding and treating human disease [pg. 2] ...In many cases, though, there will be no substitute for whole-animal studies because of the involvement of multiple tissue and organ systems in both normal and aberrant physiological conditions [pg. 15].}}
* {{cite web| title = Statement of the Royal Society's position on the use of animals in research| author = Royal Society of Medicine| date = 24 May 2023| url = https://royalsociety.org/about-us/what-we-do/supporting-researchers/animal-testing/|quote=At present the use of animals remains the only way for some areas of research to progress.}}</ref> and is used with the aim of solving medical problems such as Alzheimer's disease,<ref name="geula1998">{{cite journal |last1=Guela |first1=Changiz |last2=Wu |first2=Chuang-Kuo |last3=Saroff |first3=Daniel |last4=Lorenzo |first4=Alfredo |last5=Yuan |first5=Menglan |last6=Yankner |first6=Bruce A. |title=Aging renders the brain vulnerable to amyloid β-protein neurotoxicity |journal=Nature Medicine |date=July 1998 |volume=4 |issue=7 |pages=827–831 |doi=10.1038/nm0798-827 |pmid=9662375 }}</ref> AIDS,<ref name="AIDS2005">{{cite journal |last1=Van Rompay |first1=KK |title=Antiretroviral drug studies in nonhuman primates: a valid animal model for innovative drug efficacy and pathogenesis experiments |journal=AIDS Reviews |date=April 2005 |volume=7 |issue=2 |pages=67–83 |pmid=16092501 |url=http://www.aidsreviews.com/files/2005_7_2_67_83.pdf |archive-url=https://web.archive.org/web/20081217131711/http://www.aidsreviews.com/files/2005_7_2_67_83.pdf |archive-date=17 December 2008 }}</ref><ref>[http://www.thebody.com/cdc/tb165.html PMPA blocks SIV in monkeys]</ref><ref>[http://www.thebody.com/bp/dec99/medical.html PMPA is tenofovir]</ref> multiple sclerosis,<ref name="jameson1994">{{cite journal |last1=Jameson |first1=Bradford A. |last2=McDonnell |first2=James M. |last3=Marini |first3=Joseph C. |last4=Korngold |first4=Robert |title=A rationally designed CD4 analogue inhibits experimental allergic encephalomyelitis |journal=Nature |date=April 1994 |volume=368 |issue=6473 |pages=744–746 |doi=10.1038/368744a0 |pmid=8152486 |bibcode=1994Natur.368..744J }}</ref> spinal cord injury, many headaches,<ref name="lyuksyutova1984">{{cite journal | last1 = Lyuksyutova | first1 = AL | last2 = Lu C-C | first2 = Milanesio N | year = 2003 | title = Anterior-posterior guidance of commissural axons by Wnt-Frizzled signaling | journal = Science | volume = 302 | issue = 5652| doi=10.1126/science.1089610 | pmid=14671310 | last3 = Milanesio | first3 = N | last4 = King | first4 = LA | last5 = Guo | first5 = N | last6 = Wang | first6 = Y | last7 = Nathans | first7 = J | last8 = Tessier-Lavigne | first8 = M | last9 = Zou | first9 = Y | display-authors = 8| pages = 1984–8| bibcode = 2003Sci...302.1984L }}</ref> and other conditions in which there is no useful ''[[in vitro]]'' model system available.

==Selection==
Models are those organisms with a wealth of biological data that make them attractive to study as examples for other [[species]] and/or natural phenomena that are more difficult to study directly. Continual research on these organisms focuses on a wide variety of experimental techniques and goals from many different levels of biology—from [[ecology]], [[behavior]] and [[biomechanics]], down to the tiny functional scale of individual [[Tissue (biology)|tissues]], [[organelle]]s and [[protein]]s. Inquiries about the DNA of organisms are classed as [[Genetics|genetic]] models (with short generation times, such as the [[Drosophila melanogaster|fruitfly]] and [[Caenorhabditis elegans|nematode]] worm), [[experimental]] models, and [[genomic]] parsimony models, investigating pivotal position in the evolutionary tree.<ref>[http://genome.wellcome.ac.uk/doc_WTD020803.html What are model organisms?<!-- Bot generated title -->] {{webarchive |url=https://web.archive.org/web/20061028072001/http://genome.wellcome.ac.uk/doc_WTD020803.html |date=October 28, 2006 }}</ref> Historically, model organisms include a handful of species with extensive genomic research data, such as the NIH model organisms.<ref>[http://www.nih.gov/science/models/ NIH model organisms] {{webarchive |url=https://web.archive.org/web/20070822041956/http://www.nih.gov/science/models/ |date=August 22, 2007 }}</ref>

Often, model organisms are chosen on the basis that they are amenable to experimental manipulation. This usually will include characteristics such as short [[Biological life cycle|life-cycle]], techniques for genetic manipulation ([[inbreeding|inbred]] strains, [[stem cell]] lines, and methods of [[Transformation (genetics)|transformation]]) and non-specialist living requirements. Sometimes, the genome arrangement facilitates the sequencing of the model organism's genome, for example, by being very compact or having a low proportion of [[junk DNA]] (e.g. [[Saccharomyces cerevisiae|yeast]], [[Arabidopsis thaliana|arabidopsis]], or [[Takifugu rubripes|pufferfish]]).<ref name="Leica">{{cite web |title=Model Organisms in Research |url=https://www.leica-microsystems.com/applications/life-science/model-organisms-in-research/#:~:text=Model%20organisms%20are%20typically%20chosen,%2C%20organ%2C%20and%20system%20level. |website=Leica Microsystems |access-date=13 October 2024}}</ref>

When researchers look for an organism to use in their studies, they look for several traits. Among these are size, [[generation time]], accessibility, manipulation, genetics, conservation of mechanisms, and potential economic benefit. As comparative [[molecular biology]] has become more common, some researchers have sought model organisms from a wider assortment of [[lineage (evolution)|lineages]] on the tree of life.

===Phylogeny and genetic relatedness===
The primary reason for the use of model organisms in research is the evolutionary principle that all organisms share some degree of relatedness and genetic similarity due to [[common ancestry]]. The study of taxonomic human relatives, then, can provide a great deal of information about mechanism and disease within the human body that can be useful in medicine.{{cn|date=December 2023}}

Various phylogenetic trees for vertebrates have been constructed using comparative [[proteomics]], genetics, genomics as well as the geochemical and fossil record.<ref>{{cite journal |last1=Hedges |first1=S. Blair |title=The origin and evolution of model organisms |journal=Nature Reviews Genetics |date=November 2002 |volume=3 |issue=11 |pages=838–849 |doi=10.1038/nrg929 |pmid=12415314 }}</ref> These estimations tell us that humans and chimpanzees last shared a common ancestor about 6&nbsp;million years ago (mya). As our closest relatives, chimpanzees have a lot of potential to tell us about mechanisms of disease (and what genes may be responsible for human intelligence). However, chimpanzees are rarely used in research and are protected from highly invasive procedures. Rodents are the most common animal models. Phylogenetic trees estimate that humans and rodents last shared a common ancestor ~80-100mya.<ref>{{cite journal |last1=Bejerano |first1=Gill |last2=Pheasant |first2=Michael |last3=Makunin |first3=Igor |last4=Stephen |first4=Stuart |last5=Kent |first5=W. James |last6=Mattick |first6=John S. |last7=Haussler |first7=David |title=Ultraconserved Elements in the Human Genome |journal=Science |date=28 May 2004 |volume=304 |issue=5675 |pages=1321–1325 |doi=10.1126/science.1098119 |pmid=15131266 |bibcode=2004Sci...304.1321B }}</ref><ref name="Chinwalla2002">{{Cite journal
| last1 = Chinwalla | first1 = A. T.
| last2 = Waterston | first2 = L. L.
| last3 = Lindblad-Toh | first3 = K. D.
| last4 = Birney | first4 = G. A.
| last5 = Rogers | first5 = L. A.
| last6 = Abril | first6 = R. S.
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| title = Initial sequencing and comparative analysis of the mouse genome
| journal = Nature
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| issue = 6915
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| year = 2002
| pmid = 12466850
| bibcode = 2002Natur.420..520W| display-authors = 29
| doi-access = free
}}</ref> Despite this distant split, humans and rodents have far more similarities than they do differences. This is due to the relative stability of large portions of the genome, making the use of vertebrate animals particularly productive.{{cn|date=December 2023}}

Genomic data is used to make close comparisons between species and determine relatedness. Humans share about 99% of their genome with chimpanzees<ref>{{Cite journal
| last1 = Kehrer-Sawatzki | first1 = H.
| last2 = Cooper | first2 = D. N.
| doi = 10.1002/humu.20420
| title = Understanding the recent evolution of the human genome: Insights from human-chimpanzee genome comparisons
| journal = Human Mutation
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}}</ref><ref>{{cite journal |last1=Kehrer-Sawatzki |first1=Hildegard |last2=Cooper |first2=David N. |title=Structural divergence between the human and chimpanzee genomes |journal=Human Genetics |date=18 January 2007 |volume=120 |issue=6 |pages=759–778 |doi=10.1007/s00439-006-0270-6 |pmid=17066299 }}</ref> (98.7% with bonobos)<ref>{{Cite journal
| last1 = Prüfer | first1 = K.
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| last28 = Mugisha
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| volume = 486
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| bibcode = 2012Natur.486..527P}}</ref> and over 90% with the mouse.<ref name="Chinwalla2002" /> With so much of the genome conserved across species, it is relatively impressive that the differences between humans and mice can be accounted for in approximately six thousand genes (of ~30,000 total). Scientists have been able to take advantage of these similarities in generating experimental and predictive models of human disease.{{cn|date=December 2023}}

== Use ==
There are many model organisms. One of the first model systems for [[molecular biology]] was the bacterium ''[[Escherichia coli]]'', a common constituent of the human digestive system. Several of the bacterial viruses ([[bacteriophage]]) that infect ''[[Escherichia coli|E. coli]]'' also have been very useful for the study of gene structure and [[gene regulation]] (e.g. phages [[Lambda phage|Lambda]] and [[Enterobacteria phage T4|T4]]). However, it is debated whether bacteriophages should be classified as organisms, because they lack metabolism and depend on functions of the host cells for propagation.<ref>{{cite journal |last1=Grada |first1=Ayman |last2=Mervis |first2=Joshua |last3=Falanga |first3=Vincent |title=Research Techniques Made Simple: Animal Models of Wound Healing |journal=Journal of Investigative Dermatology |date=October 2018 |volume=138 |issue=10 |pages=2095–2105.e1 |doi=10.1016/j.jid.2018.08.005 |pmid=30244718 |doi-access=free }}</ref>

In [[eukaryote]]s, several yeasts, particularly ''[[Saccharomyces cerevisiae]]'' ("baker's" or "budding" yeast), have been widely used in [[genetics]] and [[cell biology]], largely because they are quick and easy to grow. The [[cell cycle]] in a simple [[yeast]] is very similar to the cell cycle in [[human]]s and is regulated by [[homology (biology)|homologous]] proteins. The fruit fly ''[[Drosophila melanogaster]]'' is studied, again, because it is easy to grow for an animal, has various visible congenital traits and has a [[polytene]] (giant) chromosome in its salivary glands that can be examined under a light microscope. The [[roundworm]] ''[[Caenorhabditis elegans]]'' is studied because it has very defined development patterns involving fixed numbers of cells, and it can be rapidly assayed for abnormalities.<ref>{{Cite journal |last1=Duina |first1=Andrea A. |last2=Miller |first2=Mary E. |last3=Keeney |first3=Jill B. |date=May 2014 |title=Budding Yeast for Budding Geneticists: A Primer on the Saccharomyces cerevisiae Model System |journal=Genetics |volume=197 |issue=1 |pages=33–48 |doi=10.1534/genetics.114.163188 |issn=0016-6731 |pmc=4012490 |pmid=24807111}}</ref>

==Disease models==
{{main|Animal disease model}}
Animal models serving in research may have an existing, inbred or induced [[disease]] or injury that is similar to a human condition. These test conditions are often termed as '''animal models of disease'''. The use of animal models allows researchers to investigate disease states in ways which would be inaccessible in a human patient, performing procedures on the non-human animal that imply a level of harm that would not be considered ethical to inflict on a human.{{cn|date=March 2025}}

The best models of disease are similar in [[etiology]] (mechanism of cause) and phenotype (signs and symptoms) to the human equivalent. However complex human diseases can often be better understood in a simplified system in which individual parts of the disease process are isolated and examined. For instance, behavioral analogues of [[anxiety (mood)|anxiety]] or [[pain]] in laboratory animals can be used to screen and test new [[medication|drugs]] for the treatment of these conditions in humans. A 2000 study found that animal models concorded (coincided on true positives and false negatives) with human toxicity in 71% of cases, with 63% for nonrodents alone and 43% for rodents alone.<ref>{{cite journal |last1=Olson |first1=Harry |last2=Betton |first2=Graham |last3=Robinson |first3=Denise |last4=Thomas |first4=Karluss |last5=Monro |first5=Alastair |last6=Kolaja |first6=Gerald |last7=Lilly |first7=Patrick |last8=Sanders |first8=James |last9=Sipes |first9=Glenn |last10=Bracken |first10=William |last11=Dorato |first11=Michael |last12=Van Deun |first12=Koen |last13=Smith |first13=Peter |last14=Berger |first14=Bruce |last15=Heller |first15=Allen |title=Concordance of the Toxicity of Pharmaceuticals in Humans and in Animals |journal=Regulatory Toxicology and Pharmacology |date=August 2000 |volume=32 |issue=1 |pages=56–67 |doi=10.1006/rtph.2000.1399 |pmid=11029269 }}</ref>

In 1987, Davidson et al. suggested that selection of an animal model for research be based on nine considerations. These include {{blockquote| 1) appropriateness as an analog, 2) transferability of information, 3) genetic uniformity of organisms, where applicable, 4) background knowledge of biological properties, 5) cost and availability, 6) generalizability of the results, 7) ease of and adaptability to experimental manipulation, 8) ecological consequences, and 9) ethical implications.<ref>{{Cite journal
| last1 = Davidson | first1 = M. K.
| last2 = Lindsey | first2 = J. R.
| last3 = Davis | first3 = J. K.
| title = Requirements and selection of an animal model
| journal = Israel Journal of Medical Sciences
| volume = 23
| issue = 6
| pages = 551–555
| year = 1987
| pmid = 3312096
}}</ref>}}

Animal models can be classified as homologous, isomorphic or predictive. Animal models can also be more broadly classified into four categories: 1) experimental, 2) spontaneous, 3) negative, 4) orphan.<ref name="pmid750165">{{Cite journal
| last1 = Hughes | first1 = H. C.
| last2 = Lang | first2 = C.
| doi = 10.3109/15563657808988266
| title = Basic Principles in Selecting Animal Species for Research Projects
| journal = Clinical Toxicology
| volume = 13
| issue = 5
| pages = 611–621
| year = 1978
| pmid = 750165
}}</ref>

Experimental models are most common. These refer to models of disease that resemble human conditions in phenotype or response to treatment but are induced artificially in the laboratory. Some examples include:
* The use of [[metrazol]] (pentylenetetrazol) as an animal model of [[epilepsy]]<ref name="pmid9092952">{{cite journal | author=White HS | title=Clinical significance of animal seizure models and mechanism of action studies of potential antiepileptic drugs | journal=Epilepsia | volume=38 Suppl 1 | issue= s1 | pages=S9–17 | year=1997 | pmid=9092952 | doi=10.1111/j.1528-1157.1997.tb04523.x | doi-access=free }}</ref>
* Induction of mechanical brain injury as an animal model of [[post-traumatic epilepsy]]<ref>{{cite book |doi=10.1007/978-1-4939-3816-2_27 |chapter=Animal Models of Posttraumatic Seizures and Epilepsy |title=Injury Models of the Central Nervous System |series=Methods in Molecular Biology |year=2016 |last1=Glushakov |first1=Alexander V. |last2=Glushakova |first2=Olena Y. |last3=Doré |first3=Sylvain |last4=Carney |first4=Paul R. |last5=Hayes |first5=Ronald L. |volume=1462 |pages=481–519 |pmid=27604735 |pmc=6036905 |isbn=978-1-4939-3814-8 }}</ref>
* Injection of the [[neurotoxin]] [[6-hydroxydopamine]] to dopaminergic parts of the basal ganglia as an animal model of [[Parkinson's disease]].<ref name="pmid23175810">{{cite journal | vauthors=Halje P, Tamtè M, Richter U, Mohammed M, Cenci MA, Petersson P | title=Levodopa-induced dyskinesia is strongly associated with resonant cortical oscillations. | journal=Journal of Neuroscience| volume=32 | issue=47 | pages=16541–51 | year=2012 | pmid=23175810| pmc=6621755 | doi=10.1523/JNEUROSCI.3047-12.2012}}</ref>
* [[Immunisation]] with an auto-[[antigen]] to induce an [[immune response]] to model [[autoimmune diseases]] such as [[Experimental autoimmune encephalomyelitis]]<ref name="pmid17943249">{{cite journal |last1=Bolton |first1=C. |title=The translation of drug efficacy from in vivo models to human disease with special reference to experimental autoimmune encephalomyelitis and multiple sclerosis |journal=Inflammopharmacology |date=October 2007 |volume=15 |issue=5 |pages=183–187 |doi=10.1007/s10787-007-1607-z |pmid=17943249 }}</ref>
* Occlusion of the [[middle cerebral artery]] as an [[animal models of ischemic stroke|animal model of ischemic stroke]]<ref name="pmid12442622">{{cite book |doi=10.1007/978-3-7091-6743-4_10 |chapter=Experimental Models in Focal Cerebral Ischemia: Are we there yet? |title=Research and Publishing in Neurosurgery |year=2002 |last1=Leker |first1=R. R. |last2=Constantini |first2=S. |series=Acta Neurochirurgica. Supplement |volume=83 |pages=55–59 |pmid=12442622 |isbn=978-3-7091-7399-2 }}</ref>
* Injection of blood in the [[basal ganglia]] of [[mus musculus|mice]] as a model for [[hemorrhagic stroke]]<ref name="pmid18586227">{{cite journal |vauthors=Wang J, Fields J, Doré S | title=The development of an improved preclinical mouse model of intracerebral hemorrhage using double infusion of autologous whole blood | journal=Brain Res | volume=1222 | pages=214–21 | year=2008 | pmid=18586227 | doi=10.1016/j.brainres.2008.05.058| pmc=4725309 }}</ref><ref name="pmid18193028">{{cite journal |last1=Rynkowski |first1=Michal A |last2=Kim |first2=Grace H |last3=Komotar |first3=Ricardo J |last4=Otten |first4=Marc L |last5=Ducruet |first5=Andrew F |last6=Zacharia |first6=Brad E |last7=Kellner |first7=Christopher P |last8=Hahn |first8=David K |last9=Merkow |first9=Maxwell B |last10=Garrett |first10=Matthew C |last11=Starke |first11=Robert M |last12=Cho |first12=Byung-Moon |last13=Sosunov |first13=Sergei A |last14=Connolly |first14=E Sander |title=A mouse model of intracerebral hemorrhage using autologous blood infusion |journal=Nature Protocols |date=January 2008 |volume=3 |issue=1 |pages=122–128 |doi=10.1038/nprot.2007.513 |pmid=18193028 }}</ref>
* [[Sepsis]] and [[septic shock]] induction by impairing the integrity of barrier tissues, administering live [[pathogens]] or [[toxins]]<ref name="Mouse Models of Sepsis and Septic S">{{cite journal |last1=Korneev |first1=K. V. |title=Mouse Models of Sepsis and Septic Shock |journal=Molecular Biology |date=18 October 2019 |volume=53 |issue=5 |pages=704–717 |doi=10.1134/S0026893319050108 |pmid=31661479 |doi-access=free }}</ref>
* Infecting animals with [[pathogens]] to reproduce human [[infectious diseases]]
* Injecting animals with [[agonists]] or [[Receptor antagonist|antagonists]] of various [[neurotransmitter]]s to reproduce human [[mental disorder]]s
* Using [[ionizing radiation]] to cause [[tumors]]
* Using gene transfer to cause [[tumors]]<ref>{{cite journal |vauthors=Eibl RH, Kleihues P, Jat PS, Wiestler OD | title=A model for primitive neuroectodermal tumors in transgenic neural transplants harboring the SV40 large T antigen | journal=Am J Pathol | volume=144 | issue=3 | pages=556–564 | year=1994 | pmid=8129041 | pmc=1887088}}</ref><ref>{{cite journal |vauthors=Radner H, El-Shabrawi Y, Eibl RH, Brüstle O, Kenner L, Kleihues P, Wiestler OD | title=Tumor induction by ras and myc oncogenes in fetal and neonatal brain: modulating effects of developmental stage and retroviral dose
 | journal=Acta Neuropathologica | volume=86 | issue=5 | pages=456–465 | year=1993 | pmid=8310796 | doi= 10.1007/bf00228580
 }}</ref>
* Implanting animals with [[tumor]]s to test and develop treatments using [[ionizing radiation]]
* [[artificial selection|Genetically]] selected (such as in [[diabetes|diabetic]] [[mus musculus|mice]] also known as [[NOD mice]])<ref name="pmid15099561">{{cite journal |vauthors=Homo-Delarche F, Drexhage HA | title=Immune cells, pancreas development, regeneration and type 1 diabetes | journal=Trends Immunol. | volume=25 | issue=5 | pages=222–9 | year=2004 | pmid=15099561 | doi=10.1016/j.it.2004.02.012}}</ref>
* Various animal models for [[wikt:screening|screening]] of drugs for the treatment of [[glaucoma]]
* The use of the [[ovariectomized rat]] in [[osteoporosis]] research
* Use of ''[[Plasmodium yoelii]]'' as a model of human malaria<ref name="pmid14702631">{{cite journal |last1=Hisaeda |first1=Hajime |last2=Maekawa |first2=Yoichi |last3=Iwakawa |first3=Daiji |last4=Okada |first4=Hiroko |last5=Himeno |first5=Kunisuke |last6=Kishihara |first6=Kenji |last7=Tsukumo |first7=Shin-ichi |last8=Yasutomo |first8=Koji |title=Escape of malaria parasites from host immunity requires CD4+CD25+ regulatory T cells |journal=Nature Medicine |date=January 2004 |volume=10 |issue=1 |pages=29–30 |doi=10.1038/nm975 |pmid=14702631 }}</ref><ref name="pmid16641443">{{cite journal |vauthors=Coppi A, Cabinian M, Mirelman D, Sinnis P | title=Antimalarial activity of allicin, a biologically active compound from garlic cloves | journal=Antimicrob. Agents Chemother. | volume=50 | issue=5 | pages=1731–7 | year=2006 | pmid=16641443 | doi=10.1128/AAC.50.5.1731-1737.2006 | pmc=1472199}}</ref><ref name="pmid16569221">{{cite journal |vauthors=Frischknecht F, Martin B, Thiery I, Bourgouin C, Menard R | title=Using green fluorescent malaria parasites to screen for permissive vector mosquitoes | journal=Malar. J. | volume=5 | issue= 1 | page=23 | year=2006 | pmid=16569221 | doi=10.1186/1475-2875-5-23 | pmc=1450296 | doi-access=free }}</ref>

Spontaneous models refer to diseases that are analogous to human conditions that occur naturally in the animal being studied. These models are rare, but informative. Negative models essentially refer to control animals, which are useful for validating an experimental result. Orphan models refer to diseases for which there is no human analog and occur exclusively in the species studied.<ref name="pmid750165"/>

The increase in knowledge of the [[genome]]s of non-human [[primates]] and other [[mammals]] that are genetically close to humans is allowing the production of [[Genetic engineering|genetically engineered]] animal tissues, organs and even animal species which express human diseases, providing a more robust model of human diseases in an animal model.

Animal models observed in the sciences of [[psychology]] and [[sociology]] are often termed '''animal models of behavior'''. It is difficult to build an animal model that perfectly reproduces the [[symptom]]s of depression in patients. Depression, as other [[mental disorders]], consists of [[endophenotype]]s<ref name=endophenotype>{{cite journal | last1=Hasler | first1=G. | year=2004 | title=Discovering endophenotypes for major depression | journal=Neuropsychopharmacology | volume=29 | issue=10 | pages=1765–1781 | doi=10.1038/sj.npp.1300506| pmid=15213704 | doi-access=free }}</ref> that can be reproduced independently and evaluated in animals. An ideal animal model offers an opportunity to understand [[molecular]], [[genetics|genetic]] and [[epigenetic]] factors that may lead to depression. By using animal models, the underlying molecular alterations and the causal relationship between [[Heredity|genetic]] or environmental alterations and depression can be examined, which would afford a better insight into [[pathology]] of depression. In addition, [[animal models of depression]] are indispensable for identifying novel [[therapies]] for depression.<ref>{{cite book |doi=10.1007/7854_2010_108 |chapter=Animal Models of Depression: Molecular Perspectives |title=Molecular and Functional Models in Neuropsychiatry |series=Current Topics in Behavioral Neurosciences |year=2011 |last1=Krishnan |first1=Vaishnav |last2=Nestler |first2=Eric J. |volume=7 |pages=121–147 |pmid=21225412 |pmc=3270071 |isbn=978-3-642-19702-4 }}</ref><ref>{{cite journal |last1=Wang |first1=Qingzhong |last2=Timberlake |first2=Matthew A. |last3=Prall |first3=Kevin |last4=Dwivedi |first4=Yogesh |title=The recent progress in animal models of depression |journal=Progress in Neuro-Psychopharmacology and Biological Psychiatry |date=July 2017 |volume=77 |pages=99–109 |doi=10.1016/j.pnpbp.2017.04.008 |pmid=28396255 |pmc=5605906 }}</ref>

==Important model organisms==
{{see also | List of model organisms}}

Model organisms are drawn from all three [[Domain (biology)|domains]] of life, as well as [[virus]]es. The most widely studied [[prokaryote|prokaryotic]] model organism is ''[[Escherichia coli]]'' (''E. coli''), which has been intensively investigated for over 60 years. It is a common, [[Gram-negative bacteria|gram-negative]] gut bacterium which can be grown and cultured easily and inexpensively in a laboratory setting. It is the most widely used organism in [[molecular genetics]], and is an important species in the fields of [[biotechnology]] and [[microbiology]], where it has served as the [[host organism]] for the majority of work with [[recombinant DNA]].<ref>{{cite web | title=Bacteria | url=http://www.microbiologyonline.org.uk/about-microbiology/introducing-microbes/bacteria | publisher=Microbiologyonline | access-date=27 February 2014 | archive-date=27 February 2014 | archive-url=https://web.archive.org/web/20140227212658/http://www.microbiologyonline.org.uk/about-microbiology/introducing-microbes/bacteria | url-status=dead }}</ref>

Simple model [[eukaryote]]s include baker's yeast (''[[Saccharomyces cerevisiae]]'') and fission yeast (''[[Schizosaccharomyces pombe]]''), both of which share many characters with higher cells, including those of humans. For instance, many [[cell division]] genes that are critical for the development of [[cancer]] have been discovered in yeast. ''[[Chlamydomonas reinhardtii]]'', a unicellular [[green alga]] with well-studied genetics, is used to study [[photosynthesis]] and [[motility]]. ''C. reinhardtii'' has many known and mapped mutants and expressed sequence tags, and there are advanced methods for genetic transformation and selection of genes.<ref>{{Cite web |url=http://genome.jgi-psf.org/chlamy |title=Chlamydomonas reinhardtii resources at the Joint Genome Institute |access-date=2007-10-23 |archive-url=https://web.archive.org/web/20080723150730/http://genome.jgi-psf.org/chlamy/ |archive-date=2008-07-23 |url-status=dead }}</ref> ''[[Dictyostelium discoideum]]'' is used in [[molecular biology]] and [[genetics]], and is studied as an example of [[cell communication]], [[Cellular differentiation|differentiation]], and [[programmed cell death]].
[[File:Lightmatter lab mice.jpg|thumb|[[Laboratory mouse|Laboratory mice]], widely used in medical research]]

Among invertebrates, the [[Drosophilidae|fruit fly]] ''[[Drosophila melanogaster]]'' is famous as the subject of genetics experiments by [[Thomas Hunt Morgan]] and others. They are easily raised in the lab, with rapid generations, high [[fecundity]], few [[chromosome]]s, and easily induced observable mutations.<ref name="Encyclopedia of genetics">{{cite encyclopedia | author=James H. Sang | editor=Eric C. R. Reeve | encyclopedia=Encyclopedia of genetics | title=Drosophila melanogaster: The Fruit Fly | url=https://books.google.com/books?id=JjLWYKqehRsC&q=drosophila+eggs+day+lifetime&pg=PA157 | access-date=2009-07-01 | date=2001 | publisher=Fitzroy Dearborn Publishers, I | location=USA | page=157 | isbn=978-1-884964-34-3 }}</ref> The [[nematode]] ''[[Caenorhabditis elegans]]'' is used for understanding the genetic control of development and physiology. It was first proposed as a model for neuronal development by [[Sydney Brenner]] in 1963, and has been extensively used in many different contexts since then.<ref>{{cite book | author=Riddle, Donald L. | title=C. elegans II | publisher=Cold Spring Harbor Laboratory Press | location=Plainview, N.Y | year=1997 | isbn=978-0-87969-532-3 | url=https://www.ncbi.nlm.nih.gov/books/bv.fcgi?call=bv.View..ShowTOC&rid=ce2.TOC }}</ref><ref>{{cite journal | last=Brenner | first=S | year=1974 | title=The Genetics of ''Caenorhabditis elegans'' | journal=[[Genetics (journal)|Genetics]] | volume=77 | issue=1 | pages=71–94 | doi=10.1093/genetics/77.1.71 | pmc=1213120 | pmid=4366476}}</ref> ''C. elegans'' was the first multicellular organism whose genome was completely sequenced, and as of 2012, the only organism to have its [[connectome]] (neuronal "wiring diagram") completed.<ref>{{cite journal | last1=White | first1=J | year=1986 | title=The structure of the nervous system of the nematode Caenorhabditis elegans | journal=Philos. Trans. R. Soc. Lond. B Biol. Sci. | volume=314 | issue=1165 | pages=1–340 | pmid=22462104 | doi=10.1098/rstb.1986.0056|display-authors=etal| bibcode=1986RSPTB.314....1W | doi-access=free }}</ref><ref>{{cite magazine | last=Jabr | first=Ferris | date=2012-10-02 | title=The Connectome Debate: Is Mapping the Mind of a Worm Worth It? | url=http://www.scientificamerican.com/article.cfm?id=c-elegans-connectome | magazine=Scientific American | access-date=2014-01-18
}}</ref>

''[[Arabidopsis thaliana]]'' is currently the most popular model plant. Its small stature and short generation time facilitates rapid genetic studies,<ref name="TAIR">[http://www.arabidopsis.org/portals/education/aboutarabidopsis.jsp#hist About Arabidopsis on The Arabidopsis Information Resource page] ([[The Arabidopsis Information Resource|TAIR]])</ref> and many phenotypic and biochemical mutants have been mapped.<ref name="TAIR" /> ''A. thaliana'' was the first plant to have its [[genome]] [[DNA sequencing|sequenced]].<ref name="TAIR" />

Among [[vertebrate]]s, [[guinea pig]]s (''Cavia porcellus'') were used by [[Robert Koch]] and other early bacteriologists as a host for bacterial infections, becoming a byword for "laboratory animal", but are less commonly used today. The classic model vertebrate is currently the mouse (''[[House mouse|Mus musculus]]''). Many inbred strains exist, as well as lines selected for particular traits, often of medical interest, e.g. body size, obesity, muscularity, and voluntary [[wheel-running]] behavior.<ref>{{cite journal | last1 = Kolb | first1 = E. M. | last2 = Rezende | first2 = E. L. | last3 = Holness | first3 = L. | last4 = Radtke | first4 = A. | last5 = Lee | first5 = S. K. | last6 = Obenaus | first6 = A. | last7 = Garland Jr | first7 = T. | year = 2013 | title = Mice selectively bred for high voluntary wheel running have larger midbrains: support for the mosaic model of brain evolution | journal = [[Journal of Experimental Biology]] | volume = 216 | issue = 3| pages = 515–523 | doi=10.1242/jeb.076000| pmid = 23325861 | title-link = midbrain | doi-access = free | bibcode = 2013JExpB.216..515K }}</ref>
The rat (''[[Rattus norvegicus]]'') is particularly useful as a toxicology model, and as a neurological model and source of primary cell cultures, owing to the larger size of organs and suborganellar structures relative to the mouse, while eggs and embryos from ''[[Xenopus tropicalis]]'' and ''[[Xenopus laevis]]'' (African clawed frog) are used in developmental biology, cell biology, toxicology, and neuroscience.<ref name="wallingford">{{cite journal |last1=Wallingford |first1=John B. |last2=Liu |first2=Karen J. |last3=Zheng |first3=Yixian |title=Xenopus |journal=Current Biology |date=March 2010 |volume=20 |issue=6 |pages=R263–R264 |doi=10.1016/j.cub.2010.01.012 |pmid=20334828 |bibcode=2010CBio...20.R263W }}</ref><ref name="Harland">{{cite journal | last1=Harland | first1=R.M. | last2=Grainger | first2=R.M. | year=2011 | title=MISSING | journal=Trends in Genetics | volume=27 | issue= 12| pages=507–15 | doi=10.1016/j.tig.2011.08.003 | pmid=21963197 | pmc=3601910}}</ref> Likewise, the [[zebrafish]] (''Danio rerio'') has a nearly transparent body during early development, which provides unique visual access to the animal's internal anatomy during this time period. Zebrafish are used to study development, toxicology and toxicopathology,<ref>{{cite journal |vauthors=Spitsbergen JM, Kent ML | title=The state of the art of the zebrafish model for toxicology and toxicologic pathology research—advantages and current limitations | journal=Toxicol Pathol | volume=31 | issue=Suppl | pages=62–87 | year=2003 | pmid=12597434 | pmc=1909756 | doi=10.1080/01926230390174959}}</ref> specific gene function and roles of signaling pathways.

Other important model organisms and some of their uses include: [[T4 phage]] (viral infection), ''[[Tetrahymena thermophila]]'' (intracellular processes), [[maize]] ([[transposon]]s), ''[[Hydra (genus)|hydra]]s'' ([[Regeneration (biology)|regeneration]] and [[morphogenesis]]),<ref>{{Cite journal
 | last1 = Chapman | first1 = J. A.
 | last2 = Kirkness | first2 = E. F.
 | last3 = Simakov | first3 = O.
 | last4 = Hampson | first4 = S. E.
 | last5 = Mitros | first5 = T.
 | last6 = Weinmaier | first6 = T.
 | last7 = Rattei | first7 = T.
 | last8 = Balasubramanian | first8 = P. G.
 | last9 = Borman | first9 = J.
 | last10 = Busam | first10 = D.
 | last11 = Disbennett | first11 = K.
 | last12 = Pfannkoch | first12 = C.
 | last13 = Sumin | first13 = N.
 | last14 = Sutton | first14 = G. G.
 | last15 = Viswanathan | first15 = L. D.
 | last16 = Walenz | first16 = B.
 | last17 = Goodstein | first17 = D. M.
 | last18 = Hellsten | first18 = U.
 | last19 = Kawashima | first19 = T.
 | last20 = Prochnik | first20 = S. E.
 | last21 = Putnam | first21 = N. H.
 | last22 = Shu | first22 = S.
 | last23 = Blumberg | first23 = B.
 | last24 = Dana | first24 = C. E.
 | last25 = Gee | first25 = L.
 | last26 = Kibler | first26 = D. F.
 | last27 = Law | first27 = L.
 | last28 = Lindgens | first28 = D.
 | last29 = Martinez | first29 = D. E.
 | last30 = Peng | first30 = J.
 | title = The dynamic genome of Hydra
 | journal = Nature
 | volume = 464
 | issue = 7288
 | pages = 592–596
 | year = 2010
 | pmid = 20228792
 | doi = 10.1038/nature08830
|bibcode = 2010Natur.464..592C | display-authors = 29
 | pmc = 4479502}}</ref> [[cat]]s (neurophysiology), [[chicken]]s (development), [[dog]]s (respiratory and cardiovascular systems), ''[[turquoise killifish|Nothobranchius furzeri]]'' (aging),<ref>{{Cite journal | doi = 10.1016/j.cell.2015.01.038| pmid = 25684364| title = A Platform for Rapid Exploration of Aging and Diseases in a Naturally Short-Lived Vertebrate| journal = Cell| volume = 160| issue = 5| pages = 1013–26| year = 2015| last1 = Harel | first1 = I. | last2 = Benayoun | first2 = B. R. N. A. | last3 = Machado | first3 = B. | last4 = Singh | first4 = P. P. | last5 = Hu | first5 = C. K. | last6 = Pech | first6 = M. F. | last7 = Valenzano | first7 = D. R. | last8 = Zhang | first8 = E. | last9 = Sharp | first9 = S. C. | last10 = Artandi | first10 = S. E. | last11 = Brunet | first11 = A. | pmc=4344913}}</ref> non-human primates such as the [[rhesus macaque]] and [[Common chimpanzee|chimpanzee]] ([[hepatitis]], [[HIV]], [[Parkinson's disease]], [[cognition]], and [[vaccine]]s), and [[ferret]]s ([[SARS-CoV-2]])<ref>{{Cite journal |last1=Kim |first1=Young-Il |last2=Kim |first2=Seong-Gyu |last3=Kim |first3=Se-Mi |last4=Kim |first4=Eun-Ha |last5=Park |first5=Su-Jin |last6=Yu |first6=Kwang-Min |last7=Chang |first7=Jae-Hyung |last8=Kim |first8=Eun Ji |last9=Lee |first9=Seunghun |last10=Casel |first10=Mark Anthony B. |last11=Um |first11=Jihye |last12=Song |first12=Min-Suk |last13=Jeong |first13=Hye Won |last14=Lai |first14=Van Dam |last15=Kim |first15=Yeonjae |date=2020-05-13 |title=Infection and Rapid Transmission of SARS-CoV-2 in Ferrets |journal=Cell Host & Microbe |volume=27 |issue=5 |pages=704–709.e2 |doi=10.1016/j.chom.2020.03.023 |issn=1931-3128 |pmc=7144857 |pmid=32259477}}</ref>

===Selected model organisms===
The organisms below have become model organisms because they facilitate the study of certain characters or because of their genetic accessibility. For example, [[Escherichia coli|''E. coli'']] was one of the first organisms for which genetic techniques such as [[Transformation (genetics)|transformation]] or [[Genetic engineering|genetic manipulation]] has been developed.{{cn|date=March 2025}}

The [[genome]]s of all model species have been [[genome sequencing project|sequenced]], including their [[mitochondria]]l/[[chloroplast]] genomes. [[Model organism databases]] exist to provide researchers with a portal from which to download sequences (DNA, RNA, or protein) or to access functional information on specific genes, for example the sub-cellular localization of the gene product or its physiological role.{{cn|date=March 2025}}

{| class="wikitable"
|-
!
! Model Organism
! Common name
! Informal classification
! Usage (examples)
|-
| style="background:#ffdead;" | Virus
|[[Phi X 174]]
|ΦX174
| [[Virus]]
|evolution<ref>{{cite journal |last1=Wichman |first1=Holly A. |last2=Brown |first2=Celeste J. |title=Experimental evolution of viruses: Microviridae as a model system |journal=Philosophical Transactions of the Royal Society B: Biological Sciences |date=2010-08-27 |volume=365 |issue=1552 |pages=2495–2501 |doi=10.1098/rstb.2010.0053 |pmid=20643739 |pmc=2935103 }}</ref>
|-
| rowspan="2" style="background:#ffdead;" | Prokaryotes
| ''[[Escherichia coli]]''
|''E. coli''
| [[Bacteria]]
|bacterial genetics, metabolism
|-
| ''[[Pseudomonas fluorescens]]''
|''P. fluorescens''
| [[Bacteria]]
|evolution, adaptive radiation<ref>{{cite journal |last1=Kassen |first1=Rees |title=Toward a General Theory of Adaptive Radiation |journal=Annals of the New York Academy of Sciences |date=2009-06-24 |volume=1168 |issue=1 |pages=3–22 |doi=10.1111/j.1749-6632.2009.04574.x |pmid=19566701 |bibcode=2009NYASA1168....3K }}</ref>
|-
| rowspan="6" style="background:#ffdead;" | Eukaryotes, unicellular
| ''[[Dictyostelium discoideum]]''
|
| [[Amoeba]]
| immunology, host–pathogen interactions<ref>{{cite journal |last1=Dunn |first1=Joe Dan |last2=Bosmani |first2=Cristina |last3=Barisch |first3=Caroline |last4=Raykov |first4=Lyudmil |last5=Lefrançois |first5=Louise H. |last6=Cardenal-Muñoz |first6=Elena |last7=López-Jiménez |first7=Ana Teresa |last8=Soldati |first8=Thierry |title=Eat Prey, Live: Dictyostelium discoideum As a Model for Cell-Autonomous Defenses |journal=Frontiers in Immunology |date=2018-01-04 |volume=8 |pages=1906 |doi=10.3389/fimmu.2017.01906 |pmid=29354124 |pmc=5758549 |doi-access=free }}</ref>
|-
| ''[[Saccharomyces cerevisiae]]''
| Brewer's yeast<br>Baker's yeast
| [[Yeast]]
|cell division, organelles, etc.
|-
| ''[[Schizosaccharomyces pombe]]''
| Fission yeast
| [[Yeast]]
| cell cycle, cytokinesis, chromosome biology, telomeres, DNA metabolism, cytoskeleton organization, industrial applications<ref>[http://www.pombase.org/browse-curation/fypo-slim Fission Yeast GO slim terms | PomBase<!-- Bot generated title -->]</ref><ref name="pmid38376816">{{cite journal | vauthors = Rutherford KM, Lera-Ramírez M, Wood V | title = PomBase: a Global Core Biodata Resource-growth, collaboration, and sustainability | journal = Genetics | volume = 227 | issue = 1 | date = 7 May 2024 | pmid = 38376816 | pmc = 11075564  | doi = 10.1093/genetics/iyae007 }}</ref>
|-
| ''[[Chlamydomonas reinhardtii]]''
|
| [[Algae]]
|hydrogen production<ref>{{cite journal |last1=Batyrova |first1=Khorcheska |last2=Hallenbeck |first2=Patrick C. |title=Hydrogen Production by a Chlamydomonas reinhardtii Strain with Inducible Expression of Photosystem II |journal=International Journal of Molecular Sciences |date=2017-03-16 |volume=18 |issue=3 |page=647 |doi=10.3390/ijms18030647 |pmid=28300765 |pmc=5372659 |doi-access=free }}</ref>
|-
| ''[[Tetrahymena thermophila]]'', ''[[Tetrahymena pyriformis|T. pyriformis]]''
|
| [[Ciliate]]
|education,<ref>{{cite book |doi=10.1016/B978-0-12-385967-9.00016-5 |pmc=3587665 |chapter=Tetrahymena in the Classroom |title=Tetrahymena Thermophila |series=Methods in Cell Biology |year=2012 |last1=Smith |first1=Joshua J. |last2=Wiley |first2=Emily A. |last3=Cassidy-Hanley |first3=Donna M. |volume=109 |pages=411–430 |pmid=22444155 |isbn=9780123859679 }}</ref> biomedical research<ref>{{cite book |last1=Stefanidou |first1=Maria |chapter=The use of the protozoan Tetrahymena as a cell model |pages=69–88 |editor1-last=Castillo |editor1-first=Victor |editor2-last=Harris |editor2-first=Rodney |title=Protozoa: Biology, Classification and Role in Disease |date=2014 |publisher=Nova Science Publishers |isbn=978-1-62417-073-7 }}</ref>
|-
| ''[[Emiliania huxleyi]]''
|
| [[Plankton]]
|surface sea temperature<ref>{{cite journal |last1=Fielding |first1=Samuel R. |title=Emiliania huxleyi specific growth rate dependence on temperature |journal=Limnology and Oceanography |date=March 2013 |volume=58 |issue=2 |pages=663–666 |doi=10.4319/lo.2013.58.2.0663 |bibcode=2013LimOc..58..663F |doi-access=free }}</ref>
|-
| rowspan="3" style="background:#ffdead;" | Plants
| ''[[Arabidopsis thaliana]]''
| Thale cress
| [[Flowering plant]]
|population genetics<ref>{{cite journal |last1=Platt |first1=Alexander |last2=Horton |first2=Matthew |last3=Huang |first3=Yu S. |last4=Li |first4=Yan |last5=Anastasio |first5=Alison E. |last6=Mulyati |first6=Ni Wayan |last7=Ågren |first7=Jon |last8=Bossdorf |first8=Oliver |last9=Byers |first9=Diane |last10=Donohue |first10=Kathleen |last11=Dunning |first11=Megan |last12=Holub |first12=Eric B. |last13=Hudson |first13=Andrew |last14=Le Corre |first14=Valérie |last15=Loudet |first15=Olivier |last16=Roux |first16=Fabrice |last17=Warthmann |first17=Norman |last18=Weigel |first18=Detlef |last19=Rivero |first19=Luz |last20=Scholl |first20=Randy |last21=Nordborg |first21=Magnus |last22=Bergelson |first22=Joy |author-link22=Joy Bergelson|last23=Borevitz |first23=Justin O. |title=The Scale of Population Structure in Arabidopsis thaliana |journal=PLOS Genetics |date=2010-02-12 |volume=6 |issue=2 |pages=e1000843 |doi=10.1371/journal.pgen.1000843 |pmid=20169178 |pmc=2820523 |doi-access=free }}</ref>
|-
| ''[[Physcomitrella patens]]''
| Spreading earthmoss
| [[Moss]]
| molecular farming<ref>{{cite journal |last1=Bohlender |first1=Lennard L. |last2=Parsons |first2=Juliana |last3=Hoernstein |first3=Sebastian N. W. |last4=Rempfer |first4=Christine |last5=Ruiz-Molina |first5=Natalia |last6=Lorenz |first6=Timo |last7=Rodríguez Jahnke |first7=Fernando |last8=Figl |first8=Rudolf |last9=Fode |first9=Benjamin |last10=Altmann |first10=Friedrich |last11=Reski |first11=Ralf |last12=Decker |first12=Eva L. |title=Stable Protein Sialylation in Physcomitrella |journal=Frontiers in Plant Science |date=2020-12-18 |volume=11 |pages=610032 |doi=10.3389/fpls.2020.610032 |pmid=33391325 |pmc=7775405 |doi-access=free }}</ref>
|-
| ''[[Populus trichocarpa#Use as a model organism|Populus trichocarpa]]''
| Balsam poplar
| [[Tree]]
|drought tolerance, lignin biosynthesis, wood formation, plant biology, morphology, genetics, and ecology<ref>{{cite journal| url = https://academic.oup.com/treephys/article/33/4/357/1716508| title = Revisiting the sequencing of the first tree genome: Populus trichocarpa {{!}} Tree Physiology {{!}} Oxford Academic| journal = Tree Physiology| date = April 2013| volume = 33| issue = 4| pages = 357–364| doi = 10.1093/treephys/tps081| last1 = Wullschleger| first1 = Stan D.| last2 = Weston| first2 = D. J.| last3 = Difazio| first3 = S. P.| last4 = Tuskan| first4 = G. A.| pmid = 23100257}}</ref>
|-
| rowspan="3" style="background:#ffdead;" | Animals, nonvertebrate
| ''[[Caenorhabditis elegans]]''
|Nematode, Roundworm
| [[Worm]]
|differentiation, development
|-
| ''[[Drosophila melanogaster]]''
| Fruit fly
| [[Insect]]
|developmental biology, human brain degenerative disease<ref>{{cite journal| journal=Science Express|first1=Susan L.|last1=Lindquist|first2=Nancy M.|last2=Bonini|url=https://www.hhmi.org/news/parkinsons-disease-mechanism-discovered |title=Parkinson's Disease Mechanism Discovered |date=22 Jun 2006 |publisher=Howard Hughes Medical Institute |access-date=11 Jul 2019 }}</ref><ref>{{cite journal |last1= Kim|first1=H|last2=Raphayel |first2=A |last3=LaDow |first3=E |last4= McGurk|first4=L |last5= Weber|first5=R|last6= Trojanowski|first6=J|last7= Lee|first7=V|last8= Finkbeiner|first8=S |last9= Gitler|first9=A |last10= Bonini|first10=N |date=2014 |title=Therapeutic modulation of eIF2α-phosphorylation rescues TDP-43 toxicity in amyotrophic lateral sclerosis disease models |journal= [[Nature Genetics]]|volume=46 |issue= 2|pages= 152–60|doi= 10.1038/ng.2853|pmc= 3934366|pmid= 24336168}}</ref>
|-
| ''[[Callosobruchus maculatus]]''
| Cowpea Weevil
| [[Insect]]
|developmental biology
|-
| rowspan="9" style="background:#ffdead;" | Animals, vertebrate
| ''[[Danio rerio]]''
| Zebrafish
| [[Fish]]
|embryonic development
|-
| ''[[Mummichog|Fundulus heteroclitus]]''
| Mummichog
| [[Fish]]
| [[Behavioral endocrinology|effect of hormones on behavior]]
|-
| ''[[Nothobranchius furzeri]]''
| Turquoise killifish
| [[Fish]]
|aging, disease, evolution
|-
| ''[[Japanese rice fish|Oryzias latipes]]''
| Japanese rice fish
| [[Fish]]
|fish biology, sex determination<ref>{{cite book |doi=10.1016/B978-0-12-809633-8.03245-3 |chapter=Molecular and Chromosomal Aspects of Sex Determination |title=Reference Module in Life Sciences |year=2017 |last1=Siegfried |first1=K.R. |isbn=978-0-12-809633-8 }}
</ref>
|-
| ''[[Anolis carolinensis]]''
| Carolina anole
| [[Reptile]]
|reptile biology, evolution
|-
| ''[[Mus musculus]]''
| House mouse
| [[Mammal]]
|disease model for humans
|-
| ''[[Gallus gallus]]''
| Red junglefowl
| [[Bird]]
|embryological development and organogenesis
|-
| ''[[Australian zebra finch|Taeniopygia castanotis]]''
| Australian zebra finch
| [[Bird]]
| vocal learning, neurobiology<ref>{{cite journal |last1=Mello|first1=Claudio V. |date=2014 |title= The Zebra Finch, Taeniopygia guttata: An Avian Model for Investigating the Neurobiological Basis of Vocal Learning |journal= [[Cold Spring Harbor Protocols]]|volume=2014 |issue=12 |pages= 1237–1242|doi= 10.1101/pdb.emo084574|pmc= 4571486|pmid= 25342070}}</ref>
|-
| ''[[Xenopus laevis]]''<br>''[[Western clawed frog|Xenopus tropicalis]]''<ref>{{cite news|url=http://www.genomeweb.com//node/939634?hq_e=el&hq_m=701632&hq_l=1&hq_v=2de76155bb |title=JGI-Led Team Sequences Frog Genome |date=29 April 2010 |access-date=30 April 2010 |publisher=Genome Web |work=GenomeWeb.com |url-status=dead |archive-url=https://web.archive.org/web/20110807211657/http://www.genomeweb.com//node/939634?hq_e=el&hq_m=701632&hq_l=1&hq_v=2de76155bb |archive-date=August 7, 2011 }}</ref>
| African clawed frog<br>Western clawed frog
| [[Amphibian]]
|embryonic development
|}

==Limitations==
Many animal models serving as test subjects in biomedical research, such as rats and mice, may be selectively [[sedentary lifestyle|sedentary]], [[obese]] and [[glucose intolerance|glucose intolerant]]. This may confound their use to model human metabolic processes and diseases as these can be affected by dietary energy intake and [[exercise]].<ref>{{cite journal |vauthors=Martin B, Ji S, Maudsley S, Mattson MP | year=2010 | title="Control" laboratory rodents are metabolically morbid: Why it matters | journal=Proceedings of the National Academy of Sciences | volume=107 | pages=6127–6133 | doi=10.1073/pnas.0912955107 | pmid=20194732 | issue=14 | pmc=2852022| bibcode=2010PNAS..107.6127M | doi-access=free }}</ref> Similarly, there are differences between the immune systems of model organisms and humans that lead to significantly altered responses to stimuli,<ref>{{cite journal |last1=Mestas |first1=Javier |last2=Hughes |first2=Christopher C. W. |title=Of Mice and Not Men: Differences between Mouse and Human Immunology |journal=The Journal of Immunology |date=March 2004 |volume=172 |issue=5 |pages=2731–2738 |doi=10.4049/jimmunol.172.5.2731 |pmid=14978070 |doi-access=free }}</ref><ref>{{cite journal |last1=Seok |first1=Junhee |last2=Warren |first2=H. Shaw |last3=Cuenca |first3=Alex G. |last4=Mindrinos |first4=Michael N. |last5=Baker |first5=Henry V. |last6=Xu |first6=Weihong |last7=Richards |first7=Daniel R. |last8=McDonald-Smith |first8=Grace P. |last9=Gao |first9=Hong |last10=Hennessy |first10=Laura |last11=Finnerty |first11=Celeste C. |last12=López |first12=Cecilia M. |last13=Honari |first13=Shari |last14=Moore |first14=Ernest E. |last15=Minei |first15=Joseph P. |last16=Cuschieri |first16=Joseph |last17=Bankey |first17=Paul E. |last18=Johnson |first18=Jeffrey L. |last19=Sperry |first19=Jason |last20=Nathens |first20=Avery B. |last21=Billiar |first21=Timothy R. |last22=West |first22=Michael A. |last23=Jeschke |first23=Marc G. |last24=Klein |first24=Matthew B. |last25=Gamelli |first25=Richard L. |last26=Gibran |first26=Nicole S. |last27=Brownstein |first27=Bernard H. |last28=Miller-Graziano |first28=Carol |last29=Calvano |first29=Steve E. |last30=Mason |first30=Philip H. |last31=Cobb |first31=J. Perren |last32=Rahme |first32=Laurence G. |last33=Lowry |first33=Stephen F. |last34=Maier |first34=Ronald V. |last35=Moldawer |first35=Lyle L. |last36=Herndon |first36=David N. |last37=Davis |first37=Ronald W. |last38=Xiao |first38=Wenzhong |last39=Tompkins |first39=Ronald G. |last40=Abouhamze |first40=Amer |last41=Balis |first41=Ulysses G. J. |last42=Camp |first42=David G. |last43=De |first43=Asit K. |last44=Harbrecht |first44=Brian G. |last45=Hayden |first45=Douglas L. |last46=Kaushal |first46=Amit |last47=O'Keefe |first47=Grant E. |last48=Kotz |first48=Kenneth T. |last49=Qian |first49=Weijun |last50=Schoenfeld |first50=David A. |last51=Shapiro |first51=Michael B. |last52=Silver |first52=Geoffrey M. |last53=Smith |first53=Richard D. |last54=Storey |first54=John D. |last55=Tibshirani |first55=Robert |last56=Toner |first56=Mehmet |last57=Wilhelmy |first57=Julie |last58=Wispelwey |first58=Bram |last59=Wong |first59=Wing H |title=Genomic responses in mouse models poorly mimic human inflammatory diseases |journal=Proceedings of the National Academy of Sciences of the United States of America |date=2013-02-26 |volume=110 |issue=9 |pages=3507–3512 |doi=10.1073/pnas.1222878110 |pmid=23401516 |pmc=3587220 |bibcode=2013PNAS..110.3507S |doi-access=free }}</ref><ref name="Jubb-Young-Hume">{{cite journal |last1=Jubb |first1=Alasdair W |last2=Young |first2=Robert S |last3=Hume |first3=David A |last4=Bickmore |first4=Wendy A |title=Enhancer turnover is associated with a divergent transcriptional response to glucocorticoid in mouse and human macrophages |journal=Journal of Immunology |date=15 January 2016 |volume=196 |issue=2 |pages=813–822 |doi=10.4049/jimmunol.1502009 |pmid=26663721 |pmc=4707550 }}</ref> although the underlying principles of genome function may be the same.<ref name="Jubb-Young-Hume" /> The impoverished environments inside standard laboratory cages deny research animals of the mental and physical challenges are necessary for healthy emotional development.<ref>{{Citation|last=Lahvis|first=Garet|title=The inescapable problem of lab animal restraint|date=5 December 2019 |url=https://www.ted.com/talks/garet_lahvis_the_inescapable_problem_of_lab_animal_restraint|language=en|access-date=2020-10-26}}</ref> Without day-to-day variety, risks and rewards, and complex environments, some have argued that animal models are irrelevant models of human experience.<ref>{{cite journal |last1=Lahvis |first1=Garet P |title=Unbridle biomedical research from the laboratory cage |journal=eLife |year=2017 |volume=6 |pages=e27438 |doi=10.7554/eLife.27438 |pmid=28661398 |pmc=5503508 |doi-access=free }}</ref>

Mice differ from humans in several immune properties: mice are more resistant to some [[toxins]] than humans; have a lower total [[neutrophil]] fraction in the [[blood]], a lower [[neutrophil]] [[enzymatic]] capacity, lower activity of the [[complement system]], and a different set of [[pentraxins]] involved in the [[inflammatory process]]; and lack genes for important components of the immune system, such as [[Interleukin 8|IL-8]], [[IL-37]], [[TLR10]], [[ICAM3|ICAM-3]], etc.<ref name="Mouse Models of Sepsis and Septic S"/> Laboratory mice reared in [[specific-pathogen-free]] (SPF) conditions usually have a rather immature immune system with a deficit of [[memory T cells]]. These mice may have limited diversity of the [[microbiota]], which directly affects the immune system and the development of pathological conditions. Moreover, persistent virus infections (for example, [[Herpesviridae|herpesviruses]]) are activated in humans, but not in [[specific-pathogen-free|SPF]] mice, with [[Sepsis|septic]] complications and may change the resistance to bacterial [[coinfections]]. "Dirty" mice are possibly better suitable for mimicking human pathologies. In addition, inbred mouse strains are used in the overwhelming majority of studies, while the [[human population]] is heterogeneous, pointing to the importance of studies in interstrain hybrid, [[outbred]], and nonlinear mice.<ref name="Mouse Models of Sepsis and Septic S"/>

=== Unintended bias ===
Some studies suggests that inadequate published data in animal testing may result in irreproducible research, with missing details about how experiments are done omitted from published papers or differences in testing that may introduce bias. Examples of hidden bias include a 2014 study from [[McGill University]] in [[Montreal|Montreal, Canada]] which suggests that mice handled by men rather than women showed higher stress levels.<ref>{{cite news|url=https://www.economist.com/news/christmas-specials/21712058-evolution-scientific-mainstay-worlds-favourite-lab-animal-has-been-found|title=The world's favourite lab animal has been found wanting, but there are new twists in the mouse's tale|newspaper=The Economist|access-date=2017-01-10}}</ref><ref>{{cite journal |last1=Katsnelson |first1=Alla |title=Male researchers stress out rodents |journal=Nature |date=2014-04-28 |pages=nature.2014.15106 |doi=10.1038/nature.2014.15106 |doi-access=free }}</ref><ref>{{Cite news|url=https://www.science.org/content/article/male-scent-may-compromise-biomedical-research|title=Male Scent May Compromise Biomedical Research|date=2014-04-28|newspaper=Science {{!}} AAAS|access-date=2017-01-10}}</ref> Another study in 2016 suggested that gut [[Microbiota|microbiomes]] in mice may have an impact upon scientific research.<ref>{{Cite news|url=https://www.science.org/content/article/mouse-microbes-may-make-scientific-studies-harder-replicate|title=Mouse microbes may make scientific studies harder to replicate|date=2016-08-15|newspaper=Science {{!}} AAAS|access-date=2017-01-10}}</ref>

===Alternatives===
Ethical concerns, as well as the cost, maintenance and relative inefficiency of animal research has encouraged development of alternative methods for the study of disease. Cell culture, or ''in vitro'' studies, provide an alternative that preserves the physiology of the living cell, but does not require the sacrifice of an animal for mechanistic studies. Human, inducible [[pluripotent]] stem cells can{{fact|date=December 2023}} also elucidate new mechanisms for understanding cancer and cell regeneration. Imaging studies (such as MRI or PET scans) enable non-invasive study of human subjects. Recent advances in genetics and genomics can identify disease-associated genes, which can be targeted for therapies.

Many biomedical researchers argue that there is no substitute for a living organism when studying complex interactions in disease pathology or treatments.<ref>{{cite journal | title=FDA: Why are animals used for testing medical products? | journal=FDA| url=https://www.fda.gov/AboutFDA/Transparency/Basics/ucm194932.htm| archive-url=https://web.archive.org/web/20100908061432/http://www.fda.gov/AboutFDA/Transparency/Basics/ucm194932.htm| url-status=dead| archive-date=September 8, 2010| date=2019-06-18}}</ref><ref>{{cite web | title=Society Of Toxicology: Advancing valid alternatives | url=http://www.toxicology.org/ms/air4.asp| archive-url=https://web.archive.org/web/20130105120605/http://www.toxicology.org/ms/air4.asp| url-status=dead| archive-date=2013-01-05}}</ref>

==Ethics==
Debate about the ethical use of animals in research dates at least as far back as 1822 when the British Parliament under pressure from British and Indian intellectuals enacted the first law for animal protection preventing cruelty to cattle.<ref>[https://web.archive.org/web/20061214034848/http://homepage.tinet.ie/~pnowlan/Chapter-77.htm British animal protection legislation].</ref> This was followed by the [[Cruelty to Animals Act 1835]] and the [[Cruelty to Animals Act 1849]], which criminalized ill-treating, over-driving, and torturing animals. In 1876, under pressure from the [[National Anti-Vivisection Society]], the Cruelty to Animals Act 1849 was amended to include regulations governing the use of animals in research. This new act stipulated that 1) experiments must be proven absolutely necessary for instruction, or to save or prolong human life; 2) animals must be properly anesthetized; and 3) animals must be killed as soon as the experiment is over. Today, these three principles are central to the laws and guidelines governing the use of animals and research. In the U.S., the Animal Welfare Act of 1970 (see also [[Laboratory Animal Welfare Act]]) set standards for animal use and care in research. This law is enforced by APHIS's Animal Care program.<ref>[http://awic.nal.usda.gov/government-and-professional-resources/federal-laws/animal-welfare-act AWA policies].</ref>

In academic settings in which NIH funding is used for animal research, institutions are governed by the NIH Office of Laboratory Animal Welfare (OLAW). At each site, OLAW guidelines and standards are upheld by a local review board called the Institutional Animal Care and Use Committee (IACUC). All laboratory experiments involving living animals are reviewed and approved by this committee. In addition to proving the potential for benefit to human health, minimization of pain and distress, and timely and humane euthanasia, experimenters must justify their protocols based on the principles of Replacement, Reduction and Refinement.<ref>[http://grants.nih.gov/grants/olaw/investigatorsneed2know.pdf NIH need-to-know]</ref>

"Replacement" refers to efforts to engage alternatives to animal use. This includes the use of computer models, non-living tissues and cells, and replacement of "higher-order" animals (primates and mammals) with "lower" order animals (e.g. cold-blooded animals, invertebrates) wherever possible.<ref>[https://web.archive.org/web/20000815070936/http://www.nih.gov/science/models/ list of common model organisms approved for use by the NIH])</ref>

"Reduction" refers to efforts to minimize number of animals used during the course of an experiment, as well as prevention of unnecessary replication of previous experiments. To satisfy this requirement, mathematical calculations of statistical power are employed to determine the minimum number of animals that can be used to get a statistically significant experimental result.{{cn|date=March 2025}}

"Refinement" refers to efforts to make experimental design as painless and efficient as possible in order to minimize the suffering of each animal subject.{{cn|date=March 2025}}

==See also==
<!--most of these should be removed from here and used with references in the article's text-->
{{col div}}
* [[Animals in space]]
* [[Animal testing]]
* [[Animal testing on invertebrates]]
* [[Animal testing on rodents]]
* [[Cellular model]] (numerical), e.g., ''[[Mycoplasma genitalium]]''.
* [[Ensembl]] genome database of model organisms
* [[Generic Model Organism Database]]
* [[Genome project]]
* [[History of animal testing]]
* [[History of model organisms]]
* [[History of research on Arabidopsis thaliana|History of research]] on ''[[Arabidopsis thaliana]]''
* [[History of research on Caenorhabditis elegans|History of research]] on ''[[Caenorhabditis elegans]]''
* [[Mouse models of breast cancer metastasis]]
* [[Mouse model of colorectal and intestinal cancer]]
* [[RefSeq]] - the Reference Sequence database
{{colend}}

==References==
{{Reflist}}

== Further reading ==
* {{cite journal |last1=Marx |first1=Vivien |title=Models: stretching the skills of cell lines and mice |journal=Nature Methods |date=June 2014 |volume=11 |issue=6 |pages=617–620 |doi=10.1038/nmeth.2966 |pmid=24874573 |doi-access=free }}
* {{cite journal |last1=Goldstein |first1=Bob |last2=King |first2=Nicole |title=The Future of Cell Biology: Emerging Model Organisms |journal=Trends in Cell Biology |date=November 2016 |volume=26 |issue=11 |pages=818–824 |doi=10.1016/j.tcb.2016.08.005 |pmid=27639630 |pmc=5077642 }}
* {{cite journal |last1=Lloyd |first1=Kent |last2=Franklin |first2=Craig |last3=Lutz |first3=Cat |last4=Magnuson |first4=Terry |title=Reproducibility: Use mouse biobanks or lose them |journal=Nature |date=June 2015 |volume=522 |issue=7555 |pages=151–153 |doi=10.1038/522151a |pmid=26062496 |pmc=4636083 |bibcode=2015Natur.522..151L }}

==External links==
<!--probably [[WP:UNDUE]] - list needs pruning; refs need to be removed or used in article text-->
* [[Wellcome Sanger Institute|Wellcome Trust description of model organisms]]
* [https://orip.nih.gov/comparative-medicine/programs/vertebrate-models/ National Institutes of Health Comparative Medicine Program Vertebrate Models]
* [https://www.nigms.nih.gov/Education/Pages/modelorg_factsheet.aspx/ NIH Using Model Organisms to Study Human Disease]
* [https://grants.nih.gov/grants/policy/model_organism/ National Institutes of Health Model Organism Sharing Policy]
* [https://grants.nih.gov/grants/policy/air/why_are_animals.htm/ Why are Animals Used in NIH Research]
* [http://www.fleming.gr/ Disease Animal Models – BSRC Alexander Fleming]
* [http://emice.nci.nih.gov/ Emice] – [[National Cancer Institute]]
* [https://www.komp.org/ Knock Out Mouse Project – KOMP]
* [https://mbp.mousebiology.org/ Mouse Biology Program]
* [https://www.mmrrc.org/ Mutant Mouse Resource & Research Centers, National Institutes of Health, supported Mouse Repository]
* [http://www.rrrc.us/ Rat Resource & Research Center] – [[National Institutes of Health]], supported Rat Repository
* [https://www.mmrrc.org/about/reproducibility.php/ NIH Model Organism Research Reproducibility and Rigor]

{{Model Organisms}}
{{Developmental biology}}

{{DEFAULTSORT:Model Organism}}
[[Category:Model organisms| ]]
[[Category:Animal testing]]