Editing NTP binding site

{{More citations needed|date=July 2018}}
An '''NTP binding site''' is a type of [[binding site]] found in [[nucleoside monophosphate]] (NMP) [[kinase]]s, N can be [[adenosine]] or [[guanosine]]. A [[P-loop]] is one of the [[structural motif]]s common for [[nucleoside triphosphate]] (NTP) binding sites, it interacts with the bound nucleotide's [[phosphoryl]] groups. For the binding site to be able to bind a nucleotide, the nucleotide must be complex bound to Mg<sup>2+</sup> or Mn<sup>2+</sup>. [[Nucleotide]] binding will cause conformational changes in the protein because the P-loop will bend.

NTP binding sites play a role in [[poliovirus]] [[RNA]] replication.<ref>{{cite journal | url=http://www.jbc.org/content/272/37/23261.short | pmid=9287335 | doi=10.1074/jbc.272.37.23261 | doi-access= free| title=One of Two NTP Binding Sites in Poliovirus RNA Polymerase Required for RNA Replication | journal=Journal of Biological Chemistry | date=12 September 1997 | volume=272 | issue=37 | pages=23261–23264 | last1=Richards | first1=Oliver C. | last2=Ehrenfeld | first2=Ellie | url-access=subscription }}</ref>  On the poliovirus RNA-dependent polymerase, also known as 3D<sup>pol</sup>, there are two binding sites. Both binding sites contain lysine residues; however, only the lysine at position 61 is essential for RNA sequence elongation as part of the replication process.<ref>{{Cite journal |last1=Richards |first1=O. C. |last2=Ehrenfeld |first2=E. |date=1997-09-12 |title=One of two NTP binding sites in poliovirus RNA polymerase required for RNA replication |journal=The Journal of Biological Chemistry |volume=272 |issue=37 |pages=23261–23264 |doi=10.1074/jbc.272.37.23261 |issn=0021-9258 |pmid=9287335|doi-access= free}}</ref> Additionally, a method known as computational alanine mutagenesis, has been used to find the key portions within the amino acid sequences that majorly characterize a particular NTP binding site; these regions within the sequence are conserved.<ref>{{Cite journal |last1=Bhagavat |first1=Raghu |last2=Srinivasan |first2=Narayanaswamy |last3=Chandra |first3=Nagasuma |date=2017-05-25 |title=Deciphering common recognition principles of nucleoside mono/di and tri-phosphates binding in diverse proteins via structural matching of their binding sites: Nucleotide Binding Motifs |url=https://onlinelibrary.wiley.com/doi/10.1002/prot.25328 |journal=Proteins: Structure, Function, and Bioinformatics |language=en |volume=85 |issue=9 |pages=1699–1712 |doi=10.1002/prot.25328|pmid=28547747 |s2cid=39976832 |url-access=subscription }}</ref>

==References==
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{{DEFAULTSORT:Ntp Binding Site}}
[[Category:Enzymes]]
[[Category:Nucleosides]]
[[Category:Nucleotides]]


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