Editing Sequence-tagged site

A '''sequence-tagged site''' (or '''STS''') is a short (200 to 500 base pair) [[DNA]] sequence that has a single occurrence in the [[genome]] and whose location and base sequence are known.<ref>{{cite web | url=https://www.ncbi.nlm.nih.gov/probe/docs/techsts/ | title=Sequence-Tagged Sites (STS) }}</ref>

==Usage==
STSs can be easily detected by the [[polymerase chain reaction]] (PCR) using specific [[Primer (molecular biology)|primer]]s. For this reason they are useful for constructing [[Gene mapping|genetic and physical maps]] from sequence data reported from many different laboratories. They serve as landmarks on the developing [[Gene mapping#Physical mapping|physical map]] of a genome.

When STS [[Locus (genetics)|loci]] contain genetic polymorphisms (e.g. simple sequence length polymorphisms, [[Simple sequence length polymorphism|SSLPs]], single nucleotide polymorphisms), they become valuable genetic markers, i.e. loci which can be used to distinguish individuals.

They are used in [[shotgun sequencing]], specifically to aid [[sequence assembly]].

STSs are very helpful for detecting microdeletions in some genes. For example, some STSs can be used in screening by [[Polymerase chain reaction|PCR]] to detect microdeletions in [[Azoospermia]] (AZF) [[genes]] in [[infertile]] men.

==References==
{{Reflist}}

==External links==
* [https://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/TechSTS.shtml Sequence-Tagged Sites (STS) description on NCBI]

==See also==
*[[Simple sequence length polymorphism]]

{{DEFAULTSORT:Sequence-Tagged Site}}
[[Category:Genomics]]
[[Category:DNA]]


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