A66

A66, A 66 or A-66 may refer to:

• A66 State Route (Australia), a road in South Australia connecting Keith and Port MacDonnell
• Autopista 66 (Cuba), motorway connecting the Autopista Nacional with Sancti Spiritus
• A66 road (England), a road connecting Middlesbrough and Penrith/Workington
• A66 motorway (France), a road connecting Villefranche-de-Lauragais and Pamiers
• A66 motorway (Germany), a road connecting the Taunus and Fulda
• A66 motorway (Spain), a road connecting Gijón and Seville
• Benoni Defense, in the Encyclopaedia of Chess Openings
• HLA-A66, an HLA-A serotype