Alan Fersht

Sir Alan Roy Fersht (born 21 April 1943) is a British chemist at the MRC Laboratory of Molecular Biology, Cambridge, and an Emeritus Professor in the Department of Chemistry at the University of Cambridge.<ref>{{#invoke:citation/CS1|citation |CitationClass=web }}</ref> He was Master of Gonville and Caius College, Cambridge from 2012 to 2018.<ref name="Old Monovians">{{#invoke:citation/CS1|citation |CitationClass=web }}</ref> He works at the interface of chemistry, molecular biology and biophysics on protein science, and is sometimes described as a founder of protein engineering.<ref>{{#invoke:citation/CS1|citation |CitationClass=web }}</ref><ref>BBC: brief Fersht career summary at time of knighthood</ref>

Early life and education

Fersht was born on 21 April 1943<ref name="whoswho">Template:Who's Who</ref> in Hackney, London.<ref name="l558">{{#invoke:citation/CS1|citation |CitationClass=web }}</ref> His father, Philip, was a ladies' tailor and his mother, Betty, a dressmaker. His grandparents were Jewish immigrants from Poland, Lithuania and Belarus.Template:Citation needed He was educated at Sir George Monoux Grammar School, an all-boys grammar school in Walthamstow, London.<ref name="Old Monovians"/><ref name="whoswho"/> He was a keen chess player and was the Essex County Junior champion in 1961.<ref name="Imperial College Press">Template:Cite book</ref> He was awarded a State Scholarship to read Natural Sciences at Gonville and Caius College, Cambridge, where he obtained First Class in Pt I of the Natural Sciences Tripos in 1964, First Class in Pt II (Chemistry) in 1965 and was awarded his PhD degree in 1968.<ref name="phd">Template:Cite thesis Template:Subscription required</ref> He was President of the University of Cambridge Chess Club in 1964–65 and awarded a half blue in 1965.<ref>Template:Cite newspaper The Times</ref>

Career and research

He spent a post-doctoral year (1968–1969) at Brandeis University working under William Jencks. He returned to Cambridge in 1969 as a group leader at the MRC Laboratory of Molecular Biology until 1977 and a junior research fellow at Jesus College, Cambridge until 1972. Fersht was Wolfson Research Professor of the Royal Society and Professor of Biological Chemistry at Imperial College London from 1978 to 1988. He spent a sabbatical year at Stanford University on an Eleanor Roosevelt Fellowship of the American Cancer Society with Arthur Kornberg (1978–79). Fersht was Herchel Smith Professor of Organic Chemistry at Cambridge from 1988 to 2010. He was the Director of the Cambridge Centre for Protein Engineering from 1990 to 2010 when, on reaching the retirement age, he became an Emeritus Group Leader at the MRC Laboratory of Molecular Biology. He is a Life Fellow of Gonville and Caius College.

Fersht studied for his PhD intramolecular catalysis in the hydrolysis of aspirin as a model for catalysis by enzymes. He was recruited by David Mervyn Blow and Max Perutz at the MRC Laboratory of Molecular Biology to work on the mechanism of the enzymes that were being solved there. He became a self-taught enzymologist. According to Athel Cornish-Bowden, the last major advance in understanding steady-state kinetics was made by Fersht, who introduced for the first time a meaningful definition of specificity. Fersht pointed out that <math>k_\text{cat}/K_\mathrm{m}</math> of Michaelis-Menten kinetics is the parameter that measures the capacity of an enzyme to discriminate between substrates that are available simultaneously, and so it provides the only meaningful physiological definition of specificity.<ref>Template:Cite journal</ref> For that reason, the International Union of Biochemistry and Molecular Biology has recommended the name specificity constant for this ratio.<ref>Template:Cite journal</ref>

Fersht used measurements of <math>k_\text{cat}/K_\mathrm{m}</math> to quantify the role of binding energies in catalysis and specificity, particularly in the fidelity of selection of amino acids during protein biosynthesis by the aminoacyl-tRNA synthetases, where he formulated the double-sieve editing mechanism.<ref>Template:Cite journal</ref> Fersht was one of the first classical enzymologists to use Recombinant DNA technology, which he learned in the laboratory of Arthur Kornberg, while measuring the fidelity of DNA replication from kinetics of mutagenesis in vivo. In 1982, he began a collaboration with Sir Gregory Winter in which they were the first to engineer a mutation in a protein of known structure, the tyrosyl-tRNA synthetase.<ref>Template:Cite journal</ref> Fersht went on to pioneer protein engineering as a tool for analysing protein structure and mechanism while Winter as a tool for antibody engineering. From 1990-2010, they were respectively the Director and Deputy Director of the Centre for Protein Engineering. Fersht showed that Free-energy relationships can be applied to analyse non-covalent interactions in transition states of enzyme-catalysed reactions and infer their structures in an analogous procedure to physical chemical methods for transition states in covalent reactions by measuring changes in the kinetics and thermodynamics on small changes in the structure of reagents. This procedure of studying protein engineered mutants, named Phi value analysis, was then applied to inferring the structure of transition states of protein folding and elucidating mechanisms of protein folding.<ref>Template:Cite journal</ref><ref>Template:Cite journal</ref><ref>Template:Cite journal</ref> Phi value analysis of the folding of the single-domain protein chymotrypsin inhibitor 2 uncovered a fundamental folding mechanism, “nucleation-condensation” whereby the folding chain collapses in an extended transition state around a concomitantly formed nucleus.<ref>Template:Cite journal</ref> His interests also include protein misfolding, disease and cancer.<ref name="googlescholar"/>

Selected publications

• Structure and Mechanism in Protein Science: A Guide to Enzyme Catalysis and Protein Folding<ref>Template:Cite book</ref>
• The Selected Papers of Sir Alan Fersht: Development of Protein Engineering<ref name="Imperial College Press"/>

Awards and honours

File:L1Caius1994.jpg

Scientists sharing L1 in Caius Court, Gonville and Caius College, Cambridge, taken in 1994 (Nevill Mott, Samuel Frederick Edwards, David Tabor, David Shoenberg, Rodney Hill, and Alan Fersht)

Fersht was elected a Fellow of the Royal Society (FRS) in 1983.<ref name="royal">{{#invoke:citation/CS1|citation |CitationClass=web }}</ref> The Royal Society awarded him the Gabor Medal in 1991 for molecular biology, in 1998 the Davy Medal for chemistry, in 2008 the Royal Medal and in 2020 the Copley Medal for his development and application of methods of protein engineering to provide descriptions of protein folding pathways at atomic resolution.<ref name="TRS2020">{{#invoke:citation/CS1|citation |CitationClass=web }}</ref> He is a Foreign Associate of the United States National Academy of Sciences,<ref name=nas>{{#invoke:citation/CS1|citation |CitationClass=web }}</ref> a Foreign Member of the American Philosophical Society, a Foreign Member of the Accademia dei Lincei, Member of Academia Europaea, an Honorary Foreign Member of the American Academy of Arts and Sciences and a Fellow of the Academy of Medical Sciences (FMedSci).<ref name=fmedsci>{{#invoke:citation/CS1|citation |CitationClass=web

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Fersht holds honorary doctorates from Uppsala University (1999),<ref>{{#invoke:citation/CS1|citation |CitationClass=web }}</ref> Vrije Universiteit Brussel (1999), Weizmann Institute of Science (2004), Hebrew University of Jerusalem (2006), and Aarhus University (2008). He is an Honorary Fellow of Darwin College, Cambridge (2014) and Jesus College, Cambridge (2017).<ref name="whoswho"/>

Fersht has received many prizes and medals including: the FEBS Anniversary Prize; Novo Biotechnology Award; Charmian Medal of the Royal Society of Chemistry; Max Tishler Lecture and Prize Harvard University; The Datta Lectureship and Medal of the Federation of European Biochemical Societies; Jubilee Lecture and the Harden Medal of the Biochemical Society; Feldberg Foundation Prize, Distinguished Service Award, Miami Nature Biotechnology Winter Symposium; Christian B. Anfinsen Award of the Protein Society; Natural Products Award of the Royal Society of Chemistry, Stein and Moore Award of the Protein Society;<ref>Template:Cite journal</ref><ref>Template:Cite journal</ref> Bader Award of the American Chemical Society; Kaj Ulrik Linderstrøm-Lang Prize and Medal; Bijvoet Medal of the Bijvoet Center for Biomolecular Research of Utrecht University in 2008 and the Gilbert N. Lewis Medal University of California, Berkeley, and the Wilhelm Exner Medal in 2009.<ref>editor, ÖGV. (2015). Wilhelm Exner Medal. Austrian Trade Association. ÖGV. Austria.</ref>

In 2003 he was knighted for his pioneering work on protein science.<ref name="whoswho"/> His citation on election to the Academy of Medical Sciences reads:

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File:Alan Fersht.jpg

Personal life

Fersht married Marilyn Persell in 1966 and has one son and one daughter.<ref name=whoswho/> His recreations include chess,<ref name="chess">Template:Cite book</ref><ref>He has written an account of the history of Staunton and other chess sets. Template:Cite book</ref> horology and wildlife photography.<ref name="whoswho"/>