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Phosphoserine (abbreviated as SEP or J) is an ester of serine and phosphoric acid. Phosphoserine is a component of many proteins as the result of posttranslational modifications.<ref>Template:Cite journal</ref> The phosphorylation of the alcohol functional group in serine to produce phosphoserine is catalyzed by various types of kinases.<ref>Template:Cite journal</ref><ref>Template:Cite journal</ref> Through the use of technologies that utilize an expanded genetic code, phosphoserine can also be incorporated into proteins during translation.<ref>Template:Cite journal</ref><ref>Template:Cite journal</ref><ref>Template:Cite journal</ref>

It is a normal metabolite found in human biofluids.<ref>Template:Cite journal</ref>

Phosphoserine has three potential coordination sites (carboxyl, amine and phosphate group) Determination of the mode of coordination between phosphorylated ligands and metal ions occurring in an organism is a first step to explain the function of the phosphoserine in bioinorganic processes.<ref>Template:Cite journal</ref><ref>Template:Cite journal</ref>

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